蒙古马运动相关基因的表达量差异研究  

Characterization of Exercise Relevant Genes in Mongolian Horses

作　　者：杨丽华[1] 白东义[2] 高雅罕[2] 乌日古木拉[2] 苏日古嘎[2] 芒来[2] 

机构地区：[1]内蒙古农业大学生命科学学院,呼和浩特010018 [2]内蒙古农业大学动物科学学院,呼和浩特010018

出　　处：《畜牧兽医学报》2013年第12期1900-1906,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目(31160446);内蒙古自治区科学技术厅项目(20091404);国际科技合作交流专项(2012DFB30070)

摘　　要：通过对蒙古马运动前后与运动相关基因表达量差异的研究,了解与蒙古马耐力相关的基因。采用荧光定量PCR方法检测蒙古马臀中肌中与能量代谢和肌肉生长相关的7个基因的mRNA表达量在静止期(T0)、10 000m耐力运动刚刚结束(T1)及运动后恢复期(4h后,T2)的变化;并对mRNA表达量差异显著的基因采用Western blotting方法对其蛋白的表达进行研究。结果表明,与蒙古马静止期基因的mRNA表达量相比,10 000米的耐力运动4h后有2个基因(PPARGC1A和PDK4)的mRNA表达显著上调(P<0.05),Western blotting结果也显示这2个基因的蛋白表达量在运动后发生了增加。以上结果初步揭示了,PPARGC1A和PDK4这2个与能量代谢相关的基因和蒙古马的运动关系密切,为深入研究蒙古马的运动提供了基础数据。To understand the genes related to endurance of Mongolia horses, the differential ex- pression of genes related to exercise before and after exercise was detected in this study. The ex- pression changes of 7 genes related with energy metabolism and muscle growth were measured in Mongolian horses skeletal muscle at three stages （pre-exercise （T0）, immediately post 10 000 m endurance exercise （T1） and 4 h post exercise （T2）） by using quantitative RT-PCR. And protein expression of the genes which mRNA expression levels were significantly different at the three stages were observed using Western blotting. The results showed that mRNA expression level of two genes （PPARCIIA and PDK4）increased significantly at T2 stage than that at To stage. Western blotting re- sults also showed the protein expression of two genes after endurance sports had increased. These results reveal that the PPARGCIA and PDK4 are closely related to exercise. The above results provide some data for further study of the Mongolia horse exercise.

关 键 词：蒙古马 运动 基因表达 能量代谢 

分 类 号：S821.2[农业科学—畜牧学]