中国荷斯坦牛乳房炎易感性及抗性的全基因组关联分析  

Genome-wide Association Study for Mastitis Susceptibility and Resistance in Chinese Holsteins

作　　者：王晓[1] 解小莉[1,2] 王胜[1] 钱梦樱 马裴裴[1] 张沅[1] 孙东晓[1] 刘剑锋[1] 丁向东[1] 姜力[1] 王雅春[1] 张毅[1] 张胜利[1] 张勤[1] 俞英[1] 

机构地区：[1]中国农业大学动物科技学院,北京100193 [2]西北农林科技大学理学院,杨凌712100

出　　处：《畜牧兽医学报》2013年第12期1907-1912,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(31272420);农业部奶业体系项目(CARS-37-04B);"十二五"国家科技支持项目(2011BAD28B02);"863"重大项目(2008AA101002);教育部基本科研项目(2011JS006;Z109021306);长江学者与创新团队发展计划(IRT1191)

摘　　要：本研究首先通过SAS 9.1分析2 093头北京地区中国荷斯坦牛体细胞数(SCC)的变化规律,依据SCC将牛只划分为乳房炎易感牛(Case)及抗性牛(Control);再基于Case-control设计进行全基因组关联分析。通过染色体水平的Bonferroni校正,共检测到6个SNPs与乳房炎易感性及抗性显著相关。其中19号染色体检测出的显著SNP(ARS-BFGL-NGS-78516,P=5.200 65e-05)在其200kb范围内共有3个基因(PRKRIP1、ARHGAP23和TBX21)与炎症反应密切相关。研究结果为奶牛乳房炎易感性及抗性的分子遗传基础研究提供了数据。Abstract.. The variation trends of somatic cell count （SCC） were analyzed using SAS 9.1. According to the SCC data, in 2 093 Chinese Holsteins in Beijing all of the cows were divided into two groups, mastitis susceptive cows （case） and resistant cows （control）. Based on case-control de- sign, genome-wide association study was further performed. The results showed that six SNPs were significantly relevant to mastitis susceptibility or resistance after Bonferroni adjusting on the chromosome level. Three genes （PRKRIP1, ARHGAP23, TBX21） on BTA19 closely related to inflammation response were discovered near the significant SNP （ARS-BFGL-NGS-78516, P = 5.20e-05） within 200 kb. The results provide fundamental data for molecular genetics of mastitis resistance in dairy cattle.

关 键 词：中国荷斯坦牛 乳房炎抗性 易感性 SCC Case—control 全基因组关联分析 

分 类 号：S823[农业科学—畜牧学]