日粮低营养对母羊下丘脑KiSS-1/GPR54系统表达及相关激素分泌的影响  

Effect of Low Dietary Nutrition on Hypothalamic Expression of KiSS-1/GPR54 System and Relative Hormones Secretion in Ewes

作　　者：王军[1,2] 孙蕾[1] 周海柱[2] 张涛[3] 徐晶 娄玉杰[2] 

机构地区：[1]辽宁医学院畜牧兽医学院,锦州121001 [2]吉林农业大学动物科技学院,长春130118 [3]雪龙黑牛股份有限公司,大连116031

出　　处：《畜牧兽医学报》2013年第12期1913-1918,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：辽宁省教育厅项目(L2010255);吉林省人力资源和社会保障厅项目(2010273)

摘　　要：本研究通过分析日粮低营养对母羊下丘脑KiSS-1/GPR54系统表达及相关激素分泌的影响,明确KiSS-1/GPR54系统在日粮营养调控母羊初情期启动中的作用。选择12只体重为16kg左右的3月龄雌性小尾寒羊,随机分为2组(n=6),分别给予标准营养和低营养(对照组采食量的60%),饲喂30d后,采集下丘脑和血液,利用实时荧光定量PCR、免疫组化、ELISA和放免技术测定下丘脑KiSS-1/GPR54系统表达量及LH、胰岛素和Leptin浓度。结果表明,日粮低营养可显著降低母羊下丘脑弓状核KiSS-1基因和蛋白的表达量(P<0.01),但对GPR54基因表达量并无影响(P>0.05);日粮低营养可显著降低母羊血清中LH和胰岛素(P<0.05)浓度,极显著降低Leptin浓度(P<0.01)。结果提示,下丘脑KiSS-1/GPR54系统可通过降低KiSS-1表达来参与日粮低营养对初情期启动的调控。The present study was conducted to confirm the role of KiSS-1/GPR54 system in nutri- tion regulation of puberty onset by analyzing effect of low dietary nutrition on hypothalamic ex- pression of KiSS-1/GPR54 system and relative hormones secretion in ewes. Twelve female Small- tailed Han sheep （native breed）, around 16 kg body weigh and three months old, were randomly divided into control and feed restriction group （n=6）. Control group was fed with normal nutri- tion diet and the feed restriction group fed with low nutrition diet （60~ of food intake of control group）. Thirty days latter, ewes were killed, and hypothalamus and blood were collected for de- termining hypothalamic expression of KiSS-1/GPR54 system and secretion of LH, insulin and Leptin in ewes. The results showed that low nutrition could significantly decrease expression of KISS-1 gene and protein in arcuate nucleus of hypothalamus of ewes（P〈0.01）, however, there was no significant difference on the expression of GPR54 between two groups （P〈0.05）. Low nutrition also led to decreasing of LH, insulin（P^0.05） and Leptin（P%0.01）in serum of ewes.These results indicated that hypothalamic KiSS-1/GPR54 system may involve in low nutrition regulation of puberty onset by decreasing the expression of KISS-1 in ewes.

关 键 词：营养 KISS-1 GPR54 母羊 

分 类 号：S826[农业科学—畜牧学] S814.1[农业科学—畜牧兽医]