大黄鱼稚鱼L-蛋氨酸需要量的研究  

L-METHIONINE REQUIREMENT OF LARGE YELLOW CROAKER (PSEUDOSCIAENA CROCEA R.) LARVAE

作　　者：于海瑞[1,2] 艾庆辉[1] 麦康森[1] José Luis Zambonino-Infante Chantal Louise Cahu 

机构地区：[1]中国海洋大学教育部海水养殖重点实验室 [2]山东省高校生物化学与分子生物学重点实验室(潍坊学院),潍坊学院生物与农业工程学院 [3]Unité Mixte INRA-IFREMER de Nutrition des Poissons,IFREMER, BP 70,29280 Plouzané, France

出　　处：《水生生物学报》2013年第6期1094-1102,共9页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金(30400335);国家863高技术研究发展计划(2004AA603610);中法合作先进研究计划(PRABT01–03)资助

摘　　要：研究了12—42日龄大黄鱼(Pseudosciaena crocea R.)稚鱼蛋氨酸需要量。以白鱼粉、磷虾粉和乌贼粉作蛋白源,通过添加L-晶体氨基酸使饲料与大黄鱼卵的必需氨基酸组成一致(蛋氨酸除外),制成6种等氮(8.8%)等能(16.65 kJ/g)的微黏合饲料。L-蛋氨酸梯度依次为饲料的1.19%、1.62%、2.18%、2.65%、3.13%和3.66%,或饲料蛋白的2.17%、2.95%、3.95%、4.81%、5.70%和6.65%。以生物饵料(丰年虫无节幼体和桡足类)作对照组。每处理设3个重复,每桶(180 L)内随机放3500尾初始体重为(1.93±0.11)mg的12日龄大黄鱼稚鱼。实验为期30d。结果显示,稚鱼的成活率随饲料蛋氨酸水平的升高而升高,在2.18%蛋氨酸水平时达到最高,之后无显著变化。特定生长率(SGR)随饲料蛋氨酸水平的升高而升高,在2.18%蛋氨酸水平时达到最高,之后则呈下降趋势。对照组稚鱼的成活率和SGR最高,均显著高于蛋氨酸组(P<0.05)。各组间稚鱼体脂肪和灰分差异不显著,但体蛋白随饲料蛋氨酸水平的升高而升高,在2.65%蛋氨酸水平时达到最高,之后则稍微下降;对照组鱼体蛋白和各必需氨基酸含量均显著高于其他组(P<0.05)。经二次多项式模型分析,12—42日龄大黄鱼稚鱼的蛋氨酸需要量为饲料的2.58%或饲料蛋白的4.69%。A feeding trial was conducted to investigate the optimal dietary L-methionine level for the large yellow croaker （Pseudosciaena crocea R.） larvae from 12 to 42 days after hatching （DAH 12-42）. We designed six groups of microdiets （MDs）, which contains equal amounts of nitrogen （8.8%） and energy （16.65 KJ/g）. All six groups consisted of natural protein sources from white fish meal, squid meal, and krill meal. Each group was supplemented with crystal L-AAs （except for L-methionine） to simulate the natural AA composition in eggs of large yellow croaker. L-methionine was added into each group at the concentrations （% of diet/dietary proteins） 1.19 / 2.17, 1.62/2.95, 2.18/3.95, 2.65/4.81, 3.13/5.70 and 3.66/6.65. Live feeds （Artemia nauplii and copepods） were used as a control diet. Experiments of each diet group, including control, were repeated three times using DAH 12 larvae initially weighted （1.93 ±0.11） mg. In every experiment 3500 larvae were fed with a certain diet in a 180-L tank for 30 days. The survival rate and specific growth rate （SGR） of the larvae were measured. We observed that both the survival rate and the SGR increased as the L-methionine concentration （% of diet） was raised, and they reached the peak value at the concentration 2.18. However, higher L-methionine concentrations did not cause further increase in the survival rate. On the contrast, the SGR de- creased at higher concentrations. The survival rate and SGR of the control group （live feeds） were significantly higher than all six experimental groups （P〈0.05）. We also measured the whole-body lipid and crude protein （CP） levels of the larvae. There was no significant difference in whole-body lipid or ash content between the experimental and the control groups. There was no difference among the six experimental groups either. However, the whole-body CP increased as the L-methionine level was raised and the CP reached the peak value when the concentration of L-methionine was 2.65 （% o

关 键 词：大黄鱼 稚鱼 蛋氨酸 需要量 微颗粒饲料 

分 类 号：S963[农业科学—水产养殖]