组蛋白去甲基化酶PHD锌指蛋白8在肝细胞癌中的表达及其临床意义  

Expression of PHF8 in human hepatocellular carcinoma and its clinical significance

作　　者：魏猛[1] 郑立杰[1] 史惠文[1] 李广振[1] 张宗利[1] 

机构地区：[1]山东大学齐鲁医院普外科,济南250012

出　　处：《中华肝胆外科杂志》2013年第12期904-907,共4页Chinese Journal of Hepatobiliary Surgery

摘　　要：目的探讨组蛋白去甲基化酶PHD锌指蛋白8（PHF8）在肝细胞癌（HCC）中的表达及其与肝癌临床病理学特征和预后的关系。方法采用免疫组织化学SABC方法检测60例肝细胞癌患者肿瘤组织、相应癌旁组织以及15例正常肝组织中PHF8蛋白的表达，分析PHF8表达情况与肝癌各临床病理特征的关系。结果肝细胞癌中的PHF8表达阳性率为55．0％，明显高于相应癌旁组织及正常肝组织（阳性率分别为16．7％、6．7％）（P〈0．05）。PHF8表达与肝细胞癌的肿瘤结节大小、数目、分化程度以及TNM分期密切相关（P〈0．05）。PHF8阳性组5年无瘤生存率及总生存率均明显低于PHF8阴性组（P〈0．05）。结论PHF8表达上调在肝细胞癌的发生发展中起重要作用，与患者的预后密切相关，可为预测肝癌患者手术预后和复发提供重要的参考。Objective To investigate the expression of PHFS（PHD-finger protein 8） in human hepatocellular carcinoma （HCC） and its clinicopathologic significance. Methods The expression of PHF8 in 60 hepatocellular carcinoma samples as well as their matched paraneoplastic tissues, and 15 normal liver tissues were evaluated by immunohistochemistry. Statistical methods were used to analyse the relationship between the expression of PHF8 and the clinicopathological features of these patients. Results The PHFS-positive expression rate in the HCC samples was 55.0% and it was significantly higher than that in the paraneoplastic tissues and the normal liver tissues （16. 7%, 6.7%, respective- ly, P〈0.05）. The expression of PHF8 was closely related to tumor size, tumor nodular numbers, pathological differentiation and TNM-staging （P〈0.05 for all）. The 5-year disease free survival and overall survival in the PHFS-positive group was significantly lower than that in the negative group （P〈0.05）. Conclusions PHF8 was overexpressed in HCC samples, and its expression was closely associated with HCC clinicooathological features and prognosis of the patients.

关 键 词：肝细胞癌 组蛋白去甲基化酶 PHF8 预后 

分 类 号：R735.7[医药卫生—肿瘤]