PIWILl蛋白与DICER酶在肝细胞癌中的表达及意义  

Significance and expression of PIWIL1 protein and DICER enzyme in hepatocellular carcinoma

作　　者：范正军[1] 孙涛[1] 杨飞龙[1] 程波[1] 彭飞[1] 周闯[1] 

机构地区：[1]郑州大学第一附属医院肝胆外科、肝胆外科重点实验室,郑州450052

出　　处：《中华肝胆外科杂志》2013年第12期908-911,共4页Chinese Journal of Hepatobiliary Surgery

摘　　要：目的探讨P1WIL1蛋白与DICER酶在肝细胞癌组织中的表达及临床意义。方法免疫组化检测47例肝细胞癌标本中PIWILl与DICER的表达。蛋白印迹法检测31例肝细胞癌组织中PIWILl与DICER的表达。分析PIWIL1与DICER表达及与临床特征的关系。结果P1WIL1在癌组织中高表达，在正常肝组织中低表达（P〈O．05）。DICER在正常肝组织中高表达，在癌组织中低表达或缺失（P〈0．05）。PIWILl和DICER与肿瘤分化程度、临近组织侵犯相关（P〈O．05），PIWIL1与DICER在组织中的表达呈负相关（P〈0．05）。结论PIWILl的高表达和DICER的低表达或缺失与病理分化程度及临近组织侵犯相关，PIWILl与DICER的表达负相关。Objective To explore the expression of PIWIL1 protein and DICER enzyme in hepa tocellular carcinoma （HCC） and their significance. Methods Immunohistoehemical method was used to detect the expression of PIWIL1 and DICER in 47 cases of HCC and the adjacent HCC tissues. Western blot method was used to detect the expression of PIWIL1 and DICER in 31 cases of fresh HCC tissues and their adjacent HCC tissues. The relationship between PIWIL1 and DICER and their relationships were analysed with clinical features. 12 cases of normal liver tissues were used as control group. Results The expression of PIWIL1 was high in HCC but low in normal liver tissues （P〈0.05）. The expression of DICER was high in normal liver tissues but low in HCC （P〈0.05）. The expression of PIWII.1 was positively correlated with invasion to adjacent tissues and histological differ- entiation （P〈0.05）. The expression of DICER was negatively correlated with invasion to the adjacent tissues and histological differentiation （P〈0. 05）. There was a negative correlation between PIWIL1 and DICER （P〈0.05）. Conclusions High expression of PIWlL1 and low/missing expression of DICER was related to pathological differentiation and invasion of adjacent tissues.

关 键 词：肝细胞癌 肿瘤基因蛋白 PIWILl 核糖核酸酶Ⅲ DICER 

分 类 号：R735.7[医药卫生—肿瘤]