NCAM＋c—Kit＋胆管癌细胞的肝脏祖细胞特性研究  

Features of the NCAM＋ e-Kit＋ subset of hepatic progenitor cells in intrahepatic cholangiocarcinoma

作　　者：贺艳玲[1] 徐菁[1] 杨丽红[1] 李灵敏[1] 覃艳红[2] 陈秀花[2] 徐智芳[2] 

机构地区：[1]山西医科大学病理教研室,太原030001 [2]山西医科大学第二医院血液病研究所

出　　处：《中华肝胆外科杂志》2013年第12期934-937,共4页Chinese Journal of Hepatobiliary Surgery

基　　金：山西省回国留学人员科研基金（2011-046）;山西省留学回国人员科技活动择优资助基金（2011-762）

摘　　要：目的检测肝内胆管癌细胞系RBE中神经细胞黏附分子和干细胞因子受体阳性（NCAM＋c—Kit＋）细胞亚群的肝脏祖细胞特性。方法采用免疫磁珠法筛选NCAM＋c—Kit＋和NCAMc-KitRBE亚群，通过体外培养和体内移植实验检测NCAM＋c-Kit＋RBE细胞是否具有肝脏祖细胞特性（体外增殖、克隆形成及体内成瘤能力）。结果NCAM＋c—Kit＋RBE亚群体外增殖能力高于NCAMc—Kit-亚群（F亚群＝23．56，P〈O．01）。两亚群细胞无血清培养基培养5d后成球率分别为39．1％、7．5％，差异有统计学意义（X2=18．51，P〈O．01）。裸鼠移植瘤实验中，1×104个NCAM＋c—Kit＋细胞接种2周即可成瘤（66．7％），相同条件下NCAM—c—Kit-细胞需要1×106接种3周方可成瘤（16．7％），成瘤率差异有统计学意义（P= 0．04）。结论NCAM＋c—Kit＋RBE细胞具有肝脏祖细胞特性。NCAM联合c—Kit可作为分离、纯化胆管癌干／祖细胞的标志物。Objective To identify the features of the NCAM＋ c-Kit＋ subset of hepatic progeni- tor cells in the intrahepatic cholangiocarcinoma （ICC） cell line RBE. Method Magnetic activated cell sorting was used to isolate NCAM＋ c-Kit＋/NCAM- c-Kit- subset cells, which were tested for hepatic progenitor cell properties and proliferation, colony formation, and invasive abilities in nude mice. Re- suits The cell proliferation ability of NCAM＋ c-Kit＋ subset cells was stronger than that of NCAM c-Kit- subset cells （P〈0.01）. In serum-free condition, the number of colonies formed by NCAM＋ c- Kit＋ subset cells was more than that of NCAM c-Kit- cells （P〈0.01）. 1× 104 NCAM＋ c-Kit＋ cells were enough to form tumors in nude mice after subcutaneous inoculation for two weeks, while 1 × 106 NCAM c Kit- cells were necessary to form tumors for three weeks. The tumor formation rate of NCAM＋ c-Kit＋ cells was higher than that of NCAM c-Kit cells （P=0.04）. Conclusions It is pos- sible that NCAM＋ c-Kit＋ subset cells in RBE have the properties of hepatic progenitor cells, and NCAM combined with c Kit might be a valuable marker for isolating and purifying ICC stem/progeni- tor cells.

关 键 词：胆管肿瘤 神经细胞黏附分子类 肿瘤移植 

分 类 号：R735.8[医药卫生—肿瘤]