大蒜素和阿奇霉素治疗隐孢子虫感染小鼠的效果观察  

Therapeutic Efficacy of Allitridin and Azithromycin on Cryptosporidium Infection in Mice

作　　者：王东[1] 张媛媛[2] 

机构地区：[1]河南医学高等专科学校,病原生物学与免疫学教研室,郑州451191 [2]郑州大学第四附属医院,郑州450044

出　　处：《中国寄生虫学与寄生虫病杂志》2013年第6期477-479,共3页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：将70只昆明小鼠分为4组,健康对照组(10只)、感染对照组(20只)、大蒜素治疗组(20只)和阿奇霉素治疗组(20只)。两治疗组小鼠于感染隐孢子虫后分别灌胃大蒜素1.5 mg/d或阿奇霉素2 mg/d(均溶于1 ml生理盐水中),连续12 d。用药期间密切观察各组小鼠症状,每日计数粪便中卵囊数量。治疗结束后剖杀各组小鼠,测定空肠近端肠液分泌型免疫球蛋白A(sIgA)水平,同时HE染色观察空肠近端肠黏膜病理变化。结果两治疗组小鼠卵囊排出数量,在用药后第5天明显减少并逐渐下降,与感染对照组相比差异有统计学意义(P<0.05);小鼠肠液sIgA水平测定结果显示,大蒜素治疗组(5.16μl/ml)和阿奇霉素治疗组(3.7μl/ml)之间,差异有统计学意义(P<0.05),两治疗组较感染对照组(2.89μl/ml)明显升高(P<0.05);肠黏膜病理变化观察结果显示,感染对照组小鼠病变较重,肠黏膜充血、水肿,局部可见局灶性坏死。两治疗组仅可见少量炎细胞浸润,病变较轻。可见大蒜素和阿奇霉素对治疗隐孢子虫感染小鼠均具有一定效果,大蒜素对提高肠黏膜局部防御力效果明显。Seventy Kunming mice were randomly divided into four groups named as normal control group（10）, Cryptosporidum infected group （20）, allitridin treatment group （20） and azithromycin treatment group （20）. After infection, each mouse in allitridin treatment and azithromycin treatment groups were given 1.5 mg/d allitridin or 2 mg/d azithromycin by gavage for 12 days, respectively. The mice were observed and the oocysts shedding in fecal pellets were counted daily after treatment. The level of secretory IgA （sIgA） in intestinal fluid of proximal jejunum was determined. The pathological changes in mucous membrane of proximal jejunum were observed by HE staining. Compared with infection group, the level of oocyst shedding was considerably lower in the two treatment groups （P〈0.05）. There was a significant difference in sIgA level in intestinal fluid between allitridin treatment group （5.16 μd/ml） and azithromycin treatment group （3.7 μl/ml）（P〈 0.05）. Pathological examination of intestinal mucous membrane showed that swollen mucous membrane and focal necrosis of tissue were observed in infection group, and less inflammatory cell infiltration occurred in the two treatment groups. Allitridin and azithromycin are effective in the treatment of Cryptosporidium infection. A11itridin can enhance the defense function of intestinal mucosa.

关 键 词：隐孢子虫 大蒜素 阿奇霉素 

分 类 号：R382.319[医药卫生—医学寄生虫学]