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作 者:于静萍[1] 孙志强[1] 倪新初[1] 王坚[1] 聂斌[1] 李毅[1] 胡莉钧[1] 李栋庆[1] 孟庆红[1] 孙苏平[1]
机构地区:[1]南京医科大学附属常州第二人民医院放疗科,常州213003
出 处:《中华放射医学与防护杂志》2013年第6期584-587,共4页Chinese Journal of Radiological Medicine and Protection
基 金:江苏省卫生厅指导性科研项目(Z201220);常州市卫生局重大项目(ZD201105);常州市科技支撑社会发展项目(CE20125021)
摘 要:目的 观察沙利度胺(thalidomide,TLD)联合照射对人食管癌裸鼠移植瘤生长及血管生成的影响。方法 建立人食管鳞癌裸鼠移植瘤模型,将24只荷瘤裸鼠标号,按随机数字表随机分为健康对照组、TLD组、20 Gy组、20 Gy+TLD组,每组6只。计算肿瘤抑制率,并用免疫组织化学法检测移植瘤瘤组织血管内皮细胞生长因子(VEGF)、乏氧诱导因子-1α(HIF-1α)、微血管密度(MVD)的表达水平。结果 20 Gy+TLD组荷瘤鼠瘤体积及瘤重均小于20 Gy组,差异具有统计学意义(t=3.68、4.60,P〈0.05)。20 Gy+TLD组的抑瘤率高于其他各实验组,达到60.61%。与20 Gy组相比较,20 Gy+TLD组中裸鼠移植瘤组织中VEGF、HIF-1α蛋白表达均有所降低(t=11.82、6.63,P〈0.01);20 Gy组MVD值高于20 Gy+TLD组(t=5.92,P〈0.01)。结论 沙利度胺联合照射对移植瘤的抑瘤作用较单独照射增强,可能是沙利度胺通过调控VEGF的表达,从而影响了食管癌组织的放疗敏感性。Objective To evaluate the growth and angiogenesis inhibition effects of thalidomide combined with radiation on the nude mouse transplanted with human esophageal carcinoma cells. Methods Transplant nude mouse model of human esophageal squamous cell carcinoma was established, and 24 nude mice were randomly divided into four groups: healthy control group, thalidomide group, 20 Gy group, and 20 Gy plus thalidomide group. Tumor growth inhibition rates of different groups were calculated. The expressions of vascular epithelial growth factor (VEGF), hypoxia induced factor-1α (HIF-1α) and microvessel density (MVD) were determined by the SP immunohistochemical method. Results Tumor volume and weigth of 20 Gy+TLD group were significantly lower than those in 20 Gy group (t=3.68,4.60,P〈0.05). 20 Gy+TLD treatment yielded the highest tumor inhibitory rate of 60.61%. Both VEGF and HIF-1α expressions and MVD value of 20 Gy group were higher than those of 20 Gy+TLD group (t=11.82,6.63,5.92,P〈0.01). Conclusions Thalidomide combined with radiation has an increased tumor inhibition effect compared with radiation alone, which may be achieved through VEGF pathway, thalidomide down-regulation of VEGF expression.
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