视网膜前膜内相关炎性细胞因子的免疫组化研究  

Immunohistochemical study of related inflammatory cytokines in epiretinal membranes

作　　者：高平[1] 惠延年[2] 马吉献[2] 王文勇[3] 

机构地区：[1]武警安徽省总队医院眼科,安徽省合肥市230041 [2]第四军医大学西京医院眼科,陕西省西安市710032 [3]第四军医大学西京医院病理科,陕西省西安市710032

出　　处：《眼科新进展》2000年第6期390-392,共3页Recent Advances in Ophthalmology

基　　金：国家自然科学基金资助! (No.39470 742 )

摘　　要：目的　研究增生性玻璃体视网膜病变 (proliferative vitreoretinopathy,PVR)的视网膜前膜 (epiretinal m embranes,ERM)中炎性细胞因子白细胞介素 - 1β(interleukin- 1β,IL- 1β)、白细胞介素 - 6 (interleukin- 6 ,IL- 6 )、白细胞介素 - 8(interleukin- 8,IL- 8)和肿瘤坏死因子 - α(tum or necrosis factor- α,TNF- α)的表达及其作用。方法　用免疫组织化学方法对19例复杂性视网膜脱离行玻璃体切割术时剥离的视网膜前膜进行观察。结果　 IL- 1β、IL-6、IL- 8和 TNF- α分别在 9、12、11和 15例视网膜前膜中表达 ,尤其以细胞外基质中明显 ,4种细胞因子在 4例膜中同时表达 ,仅有 1例膜中细胞内表达 IL- 6。Objective To investigate the expression and role of inflammatory cytokines like interleukin 1β (IL 1β )、interleukin 6(IL 6)、interleukin 8(IL 8) and tumor necrosis factor alpha(TNF α)in epiretinal membranes(ERM)of eyes with proliferative vitreoretinopathy(PVR).Methods Nineteen epiretinal membranes were obtained from eyes undergoing vitrectomy for retinal detachment complicated with PVR and observed by immunohistochemical methods.Results Expression of IL 1β 、IL 6、IL 8 and TNF α were observed in 9、12、11and 15 membranes respectively, with positive staining mostly in the extracellular matrix of epiretinal membranes. Only one membrane showed positive to IL 6 intracellularly, expression for all the cytokines simultaneously in 4 membranes.Conclusion The findings indicate that cytokine mediated pathways of inflammation are involved in the pathogenesis of PVR.

关 键 词：视网膜前膜 增生性玻璃体视网膜病变 细胞因子 

分 类 号：R774.130.2[医药卫生—眼科]