E6.BARF1p调控自杀基因CD/UPRT.UL49的表达对鼻咽癌细胞的体外杀伤效应  

Lethal effect of the expression of suicide gene CD/UPRT.UL49 modulated by E6.BARF1p on nasopharyngeal carcinoma cells in vitro

作　　者：卿菁[1,2] 赵素萍[1] 蒋卫红[1] 章华[1] 

机构地区：[1]中南大学湘雅医院耳鼻咽喉科,湖南长沙410008 [2]宁波市第一医院耳鼻咽喉科,浙江宁波315000

出　　处：《中国耳鼻咽喉头颈外科》2013年第12期621-624,共4页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：国家自然科学基金资助项目(30672296)

摘　　要：目的体外实验观察及检测E6.BARF1p.CD/UPRT.UL49/5-Fc系统对鼻咽癌上皮细胞系2(nasopharyngeal carcinoma epithelial-2,CNE-2)的杀伤效应。方法用高效液相色谱法测定γ射线干预对前体药物5-氟胞嘧啶(5-fluorocytosine,5-Fc)转换效率的影响;用相对存活率表示γ射线联合前体药物5-Fc对转染自杀基因E6.BARF1p.CD/UPRT.UL49的CNE-2细胞和野生型CNE-2细胞的杀伤作用;流式细胞仪检测γ射线联合前体药物5-Fc对转染自杀基因E6.BARF1p.CD/UPRT.UL49的CNE-2细胞的杀伤作用及旁杀效应。统计分析采用SPSS10.0软件进行t检验及方差分析,P<0.05表示有统计学意义。结果转染自杀基因E6.BARF1p.CD/UPRT.UL49的CNE-2细胞在接受放射治疗联合前体药物5-Fc的作用后,其生长受到不同程度的抑制。即使仅有10%的CNE-2细胞成功转染了自杀基因E6.BARF1p.CD/UPRT.UL49,亦可因旁杀效应而杀死37.46%的肿瘤细胞。结论 E6.BARF1p.CD/UPRT.UL49/5-Fc自杀基因/前体药物系统对鼻咽癌CNE-2细胞具有直接杀伤和旁杀效应。OBJECTIVE To observe and detect the lethal effect of the expression of E6.BARFlp. CD/UPRT.UL49/5-FC on nasopharyngeal carcinoma CNE-2 cells in vitro. METHODS MTT method was used to detect the conversion efficiency of prodrug 5-Fc interfered by different quantity of yradiation. The lethal effect of γ, radiation combined with prodrug 5-Fc on wild nasopharyngeal carcinoma CNE-2 cells and nasopharyngeal carcinoma CNE-2 cells transfected with E6.BARF1p.CD/UPRT.UL49 was represented by relative survival rate. The lethal and bystander effect of yradiation combined with prodrug 5-Fc on wild nasopharyngeal carcinoma CNE-2 cells and nasopharyngeal carcinoma CNE-2 cells transfected with E6.BARF1p.CD/UPRT.UL49 was detected by flow cytometry （FCM） . T test and ANOVA were used to analyze the differences and P〈0.05 means significant. RESULTS The growth of CNE- 2 cells expressing suicide gene was suppressed more or less after being treated withyradiation combined with prodrug 5-Fc. Although there were only 10% of the CNE-2 cells transfected with E6.BARF1p.CD/UPRT.UL49 gene, yet 37.46% tumor cells were killed via bystander effect. CONCLUSION The system of E6.BARFlp.CD/UPRT. UL49/5-FC has direct cytotoxic and bystander effect on nasopharyngeal carcinoma CNE-2 cells.

关 键 词：细胞 培养的 基因 转基因 自杀 细胞凋亡 流式细胞术 旁杀效应 

分 类 号：R739.63[医药卫生—肿瘤]