苦参碱对白血病细胞NKG2D配体表达的作用研究  

Effects of Matrine on the Expression of NKG2D Ligands in Leukemia Cells

作　　者：马玲娣[1] 卢绪章[2] 朱志超[1] 蒋丽佳[1] 周民[2] 钱思轩[3] 李建勇[3] 

机构地区：[1]南京医科大学附属常州市第二人民医院中心实验室,江苏常州213000 [2]南京医科大学附属常州市第二人民医院血液科,江苏常州213000 [3]南京医科大学第一附属医院、江苏省人民医院血液科,江苏南京210029

出　　处：《中国实验血液学杂志》2013年第6期1429-1434,共6页Journal of Experimental Hematology

基　　金：国家自然科学基金(编号:81101647);南京医科大学博士后课题

摘　　要：本研究旨在探讨人白血病细胞表面NK细胞活化性受体NKG2D配体分子的表达水平及中药苦参碱对白血病细胞NKG2D配体表达的作用。流式细胞术检测白血病细胞表面MICA/B和ULBP1、2、3四种NKG2D配体的表达。苦参碱处理白血病细胞株K562、OUN-1、U937和K562/AO2及原代培养的人白血病细胞,流式细胞术分析药物处理后细胞表面NKG2D配体的表达改变。结果表明,几株常见人白血病细胞及原代人白血病细胞表面均有NKG2D配体分子的表达,多数有ULBP分子的高表达或ULBP表达明显高于MICA/B,但不同细胞表面NKG2D配体的表达模式明显不同。苦参碱处理可上调白血病细胞表面部分NKG2D配体的表达水平,提示苦参碱对不同白血病细胞NKG2D配体分子的表达调节不同。K562细胞表面NKG2D配体ULBP2和ULBP3分子的高表达可能是介导NK细胞对其高杀伤性的重要原因。结论:人白血病细胞及原代人白血病细胞表面均有NKG2D配体的表达,但不同细胞NKG2D配体的表达模式不同。苦参碱可上调白血病细胞表面部分NKG2D配体分子的表达。This study was aimed to analyze the expression of NKG2D ligands in human leukemic cells and to investigate the effects of matrine on NKG2D ligand expression. The expressions of NKG2D ligand MICA/B, ULBP1-3 in several human leukemia cell lines （K562, OUN-1, U937 and K562/AO2 ）, as well as primary leukemic cells isolated from malignant leukemia patients were analyzed by flow cytometry. After treatment with different doses of matrine, the expression level of NKG2D ligands in these leukemic cells was detected by FCM. The results indicated that NKG2D ligand expression was detected in both the leukemia cell lines and primary maiignant leukemic cells. G-enerally, the expression of ULBP was high or obviously higher than that of MICA/B in leukemia cell lines and primary leukemic cells. The expression pattern of NKG2D ligands was different among these cells, possibly due to the different types of leukemia. Not all the expression of NKG2D ligands was upregulated after matrine treatment. Much higher expressions of ULBP2 and ULBP3 were found in K562 cells, compared to the other ceU lines, which partly contributes to the higher sensitivity of K562 cells to NK cytotoxicity as target cells. It is concluded that there is universal expression of NKG2D ligand in leukemia cells. The high ULBP expression is prevalent in human leukemia cells. Matrine has the potential to induce the expression of NKG2D ligands in leukemia cells.

关 键 词：苦参碱 白血病 NKG2D配体 NK细胞 

分 类 号：R733[医药卫生—肿瘤] R979.1[医药卫生—临床医学]