宫颈癌宫颈活检组织DAPK1基因启动子甲基化的临床意义  

Clinical significance of cervical biopsies' DAPK1 gene promoter methylation in cervical carcinoma

作　　者：沈婷[1] 雷晓真 刘凌芝[1] 李俊华[2] 张婧[3] 

机构地区：[1]江西省妇幼保健院产科,江西南昌330006 [2]湖北省襄阳市中心医院儿科,湖北襄阳441021 [3]湖北省襄阳市中心医院妇产科,湖北襄阳441021

出　　处：《中国现代医生》2013年第35期16-17,共2页China Modern Doctor

摘　　要：目的研究宫颈活检组织DAPK1基因甲基化在宫颈癌中的临床意义。方法采用甲基化特异性PCR检测宫颈癌患者和正常女性宫颈活检组织DAPK1基因启动子甲基化,比较其差异并分析宫颈癌患者宫颈活检组织DAPK1基因启动子甲基化与临床病理因素的关系。结果宫颈癌患者宫颈活检组织DAPK1基因启动子甲基化率显著高于正常女性(P<0.05)。宫颈癌患者宫颈活检组织DAPK1基因启动子甲基化率在年龄、吸烟史、酗酒史和病理类型无明显差异(P>0.05),在HPV感染、分化程度、肿瘤直径、淋巴结转移、远处转移和Figo分期中的差异具有统计学意义(P<0.05)。结论宫颈癌患者宫颈活检组织DAPK1基因启动子甲基化率显著高于正常人,与病情及预后密切相关。Objective To study the clinical significance of cervical biopsies＇ DAPK1 gene promoter methylation in cervical carcinoma. Methods Methylation specific PCR was used to detect DAPK1 gene promoter＇s methylation in cervical biopsies from cervical carcinoma and healthy female. The differences were compared. The relationship between clinic pathological factors and DAPK1 gene promoter＇s methylation was analyzed. Results The methylation rate in cervical biopsies from cervical carcinoma patients was significantly higher than in healthy female （P 〈 0.05）. The differences of DAPK1 gene＇s methylation rates in HPV infection, histology differentiation, tumor diameters, lymph node metastasis, metastasis and Figo staging were statistics significant （P 〈 0.05）, while they were not in age, smoking history, history of alcohol abuse and pathological types. Conclusion The methylation rate in cervical biopsies from cervical carcinoma patients is significantly higher than in healthy female, which could be served as a tumor biomarker for condition and prognosis evaluation of cervical carcinoma.

关 键 词：宫颈癌 DAPK1 甲基化 

分 类 号：R737.33[医药卫生—肿瘤]