广东地区胃癌患者中医临床证候分型与miR-449表达相关性研究  

Study on TCM Syndrome and Expression of MiR-449 in Gastric Carcinoma Tissue

作　　者：李立平[1,2] 吴炜景[1] 邱娴[1] 李晓[2] 赵亚刚[1] 

机构地区：[1]广州军区总医院消化内科,广东广州510010 [2]广州中医药大学第一临床医学院,广东广州510405

出　　处：《中华中医药学刊》2013年第12期2695-2698,I0026,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：广东省科技计划资助项目(2011B031800317);广东省大学生创新实验资助项目(1057212046)

摘　　要：目的:研究不同中医症候临床分型胃癌患者癌变组织miR-449表达差异,探讨胃癌患者中医证候临床分型与miR-449表达情况的相关性。方法:收集2011年1月—2012年10月就诊于广州军区广州总医院消化内科及广州中医药大学第一附属医院脾胃病科的患者,其中以"胃脘痛"为中医诊断并确断为"胃癌"患者60名为研究对象,中医四诊对其进行中医辨证分型;胃镜下钳取胃癌组织及癌旁周围正常组织为标本,应用实时荧光定量PCR(qRT-PCR)检测标本miR-449表达情况。结果:①60名患者中医证型以"脾胃湿热型"为主,占46.67%,明显高于其他证型比率。②相对于癌旁正常组织,所有患者胃癌组织miR-449表达下调,相对表达量为32.367±7.318(P=0.001)。③"脾胃湿热型"患者胃癌组织miR-449相对表达量为26.250±2.287,明显低于其他证型(P<0.05)。结论:广东地区胃癌患者中医症候临床分型以"脾胃湿热证"为主,"脾胃湿热证"患者胃癌组织内miR-449表达下调,表明湿热之邪可能通过诱导组织miR-449表达下调参与胃癌的发生与发展。Objective： To study the different expression of miR -449 in the gastric carcinoma tissue from the patients with gastric cancer, and discuss the relationship between the TCM syndrome and expression of miR -449 in gastric carci- noma tissue. Methods： We selected 60 patients with epigastralgia being analyzed according to syndrome differentiation, who visited the Guangzhou General Hospital of Guangzhou Military command or the First Affiliated Hospital of Guangzhou University of Chinese Medicine during January 2011 to October 2012. The gastric carcinoma tissues and tumor - adjacent normal tissues were taken by electronic gastroscopy, and the Quantitative Real Time PCR was performed to detect the ex- pression of miR-449. Results：There were 46.67% patients with damp -heat in spleen and stomach type, which was significantly higher than other type＇ s. In gastric carcinoma tissue, the expression level of miR - 449 was lower than the one in the tumor - adjacent normal tissue （2 -aAct = 32. 367 ±7. 318, P = 0.009）. Comparing with other type＇ s, the ex- pression level of miR-449 in the patients with damp -heat in spleen and stomach type was lower （2-ZAC, = 26. 250 ±2. 287, P 〈 0. 05 ）. Conclusion：The primary TCM syndrome of the patients with gastric cancer is damp -heat in spleen and stomach in Guangdong area. We also find that the expression level of miR - 449 in the patients with damp - heat in spleen and stomach type was lower. It demonstrates that the heat pathogen and damp evil may contribute to the happening and develooment of gastric cancer by reducing the expression of miR -499.

关 键 词：MICRORNA MiR-449 中医证型 胃癌 胃脘痛 

分 类 号：R222.19[医药卫生—中医基础理论]