可溶性syndecan-1对人多发性骨髓瘤细胞体外的生物学作用  

The biological role of soluble syndecan 1 against human multiple myeloma cells in vitro

作　　者：李新燕[1] 陆肇阳 Klein B 张学光[1] 

机构地区：[1]苏州医学院免疫学教研室,苏州215007 [2]法国蒙彼利埃总医院细胞与基因治疗中心

出　　处：《中国免疫学杂志》2000年第12期633-637,共5页Chinese Journal of Immunology

基　　金：国家自然科学青年科学基金资助 !(3970 0 130 )

摘　　要：目的 :研究可溶性syndecan 1(CD138)分子对人多发性骨髓瘤 (MM)细胞生长行为的影响及其机制。方法 :用亲和层析法分离可溶性syndecan 1;借助3H TdR掺入、间接免疫荧光、ANNEXIN V及PI标记分析MM细胞增殖、凋亡及周期变化。结果 :①从XG 2细胞培养上清中分离纯化得到分子量为 6 0kD左右的可溶性syndecan 1分子 ;②可溶性syndecan 1分子在体外能抑制XG 1增殖 ,阻滞细胞增殖停留在G2 /M期 ,并介导其凋亡 ;FGF、5 %小牛血清、肝素酶III可逆转一定浓度可溶性syn decan 1的作用 ,HGF、VEGF和IGF 1可部分逆转可溶性syndecan 1分子对细胞的生长抑制作用 ;③IL 6及激发型抗IL 6R130(gp130 )单抗对可溶性syndecan 1作用无影响。结论 :可溶性syndecan 1分子通过不同于IL 6及其受体的信号传导途径 。Objective:To explore the effects and mechanisms of soluble syndecan 1 on the behavior of human multiple myeloma (MM) cells Methods:To purify soluble syndecan 1 from the supernatants of XG 2 cell line using chromatography; Through 3 H TdR incorporation, Annexing V and PI staining, we studied the biological role of purified soluble syndecan 1 on human myeloma cells in vitro Results:① We obtained the product (molecular weight 60 kD )from cultured supernatants of XG 2 which recognized by specific anti syndecan 1 antibody B B4;②Purified soluble syndecan 1 inhibited the growth ,arrested cell cycle in M phase and induced apoptosis of XG 1 cells in a dose dependent fashion in vitro, but heparitinase, fetal calf serum and fibroblast growth factor can reverse completely the effects of soluble syndecan 1, HGF,VEGF,IGF 1 reverse it partially;③IL 6 and anti gp130 agonistic monoclonal antibodies did not show any effects on soluble syndecan 1 Conclusion:Soluble syndecan 1 participated in the regulation cell growth of MM as a competitive inhibitors and a negative regulator through one signal transduction that is different from IL 6

关 键 词：多发性骨髓瘤 可溶性syndecan-1 细胞凋亡 

分 类 号：R733.3[医药卫生—肿瘤]