多不饱和脂肪酰乙醇胺的合成工艺研究  

Synthesis of polyunsaturated fatty acyl ethanolamine

作　　者：方华[1] 谢全灵[1] 黄晓燕[1] 晋文慧[1] 易瑞灶[1] 洪专[1] 

机构地区：[1]国家海洋局海洋生物资源综合利用工程技术研究中心,福建厦门361005

出　　处：《中国油脂》2013年第12期53-56,共4页China Oils and Fats

基　　金：海洋公益性行业科研专项经费项目(201205022-8);国家海洋局青年基金(2012537);厦门市重大科技创新平台项目(3502Z20111001)

摘　　要：采用藻油甘油酯为起始原料,通过皂化和酰化反应制备多不饱和脂肪酰乙醇胺。研究了草酰氯用量、催化剂N,N-二甲基甲酰胺(DMF)用量、缚酸剂三乙胺用量、反应时间和物料摩尔比对反应转化率的影响,并用单因素实验对工艺条件进行了优化。得到的最佳反应条件为:多不饱和脂肪酸与乙醇胺摩尔比1∶1.2(多不饱和脂肪酸8.0 g,乙醇胺1.8 g)、草酰氯2.4 mL、DMF 1.6 mL、三乙胺6.0 g、反应时间1 h(冰水浴中反应)。在最佳反应条件下转化率达到93.45%。反应粗产物采用硅胶柱层析技术进行分离纯化,对制备的多不饱和脂肪酰乙醇胺产物主要成分进行了红外光谱以及高效液相色谱的初步鉴定,制备产物中多不饱和脂肪酰乙醇胺的总含量为97.96%。Polyunsaturated fatty acyl ethanolamine was prepared by saponification and acylation of algae oil glyeeride. The effects of oxalyl chloride dosage, N, N - dimethylformaide （DMF） dosage, triethyl- amine dosage, reaction time and molar ratio of reaction materials on conversion rate were studied. The optimal conditions were obtained by single factor experiment as follows ： molar ratio of polyunsaturated fat- ty acid to ethanolamine 1 ： 1.2 （ 8.0 g of polyunsaturated fatty ride dosage 2.4 mL, DMF dosage 1.6 mL and triethylamine acid, 1.8 g of ethanolamine）, oxalyl chlo- dosage 6.0 g, reaction time 1 h （ in cooling water）. The conversion rate reached 93.45% under the optimal conditions. The crude product was puri- fied by silica gel column chromatography, and the main components of polyunsaturated fatty acyl ethanola- mine were preliminarily identified by FT - IR and HPLC, and the total content of polyunsaturated fatty acyl ethanolamine in the product was 97.96%.

关 键 词：多不饱和脂肪酰乙醇胺(PUFAE) 单因素实验 酰化反应 

分 类 号：TS221[轻工技术与工程—粮食、油脂及植物蛋白工程]