奥沙利铂或5-FU对人肝癌HepG2细胞低氧低血清损伤后的增殖的影响  

Effect of Oxaliplatin or 5-FU on Proliferation of Human Hepatoma Cell HepG2 after Hypoxia and Serum Limitation

作　　者：吕俊丽[1] 刘志佳[1] 刘玮璐[1] 李玉莲[1] 

机构地区：[1]哈尔滨医科大学附属第三医院科研科,黑龙江哈尔滨150081

出　　处：《现代生物医学进展》2013年第33期6460-6462,共3页Progress in Modern Biomedicine

摘　　要：目的:探讨奥沙利铂或5-FU单药对经低氧低血清损伤的人肝癌HepG2细胞增值的抑制作用。方法:体外培养HepG2细胞,建立低氧低血清细胞模型,分组选用奥沙利铂、5-FU、奥沙利铂+低氧、5-FU+低氧、奥沙利铂+低血清、5-FU+低血清、奥沙利铂+低氧低血清、5-FU+低氧低血清,48 h后MTT法检测不同浓度的奥沙利铂或5-FU对低氧、低血清和低氧低血清组的细胞增殖情况。结果:奥沙利铂或5-Fu对HepG2细胞的抑制作用呈明显的剂量依赖关系(P<0.05),细胞低氧低血清损伤同时奥沙利铂或5-FU处理后的抑制率明显高于单独用药组(P<0.01)。结论:奥沙利铂或5-FU可抑制HepG2细胞的增殖,低氧低血清处理后,抑制作用更强。Objective： To investigate the growth inhibition effects of oxaliplatin or 5-FU on HepG2 after hypoxia and serum limitation. Methods： The HepG2 cells were treated with different concentrations of oxaliplatin or 5-FU, cells injured model in vitro were induced by hypoxia and low serum. The HepG2 cells in logarithmic phase were respectively treated with oxaliplatin, 5-FU, oxaliplatin＋h- ypoxia, 5-FU＋hypoxia, oxaliplatin＋serum limitation, 5-FU＋serum limitation, oxaliplatin＋hypoxia＋serum limitation, 5-FU＋hypoxia＋serum limitation, the growth inhibition rate of HepG2 ceils was detected by MTT method after 48 h. Results： Oxaliplatin or 5-FU could inhibit the proliferation of HepG2 cells and the inhibition depended on the exposure dose （P 〈0.05）. After the induced of hypoxia and low serum, the inhibition rate were significantly increased as compared with those of the cells treated with oxaliplatin or 5-FU alone （P〈 0.01）. Conclusions： Oxaliplatin or 5-FU can enhance the inhibition rate of HepG2 cells injured by hypoxia and serum limitation.

关 键 词：奥沙利铂 5-FU 低氧低血清 细胞增殖 

分 类 号：R735.7[医药卫生—肿瘤]