多甲氧基黄酮对人胶质母细胞瘤细胞系生物学特性的影响  

Effect of Polymethoxylated Flavonoids on Biological Behavior of Human Glioblastoma Cells

作　　者：车震[1] 康娜[1] 郝东宁[1] 马瑞[1] 宋彦彬[1] 

机构地区：[1]榆林市第一医院神经外科,陕西榆林718000

出　　处：《现代生物医学进展》2013年第33期6471-6474,共4页Progress in Modern Biomedicine

摘　　要：目的:多甲氧基黄酮(PMFs)因其抗氧化、抗癌、抗炎及抗动脉粥样硬化等多样的生物学活性受到国内外学者的广泛关注,但其在神经胶质瘤治疗中的潜在作用尚未见报道。本研究对多甲氧基黄酮处理人胶质母细胞瘤细胞系对其生物学特性的影响,初步探讨PMFs在神经胶质瘤治疗中的潜在应用。方法:不同浓度(0,20,40,60,80,100μg/mL)的PMFs处理人胶质母细胞瘤细胞系U251不同时间后,分别用Annexin V/PI双染法检测细胞凋亡的变化,MTT法检细胞活力的变化,Transwell小室实验检测细胞侵袭能力的变化。结果:随着多甲氧基黄酮的浓度及处理时间的增加,细胞的增殖明显受到抑制,同时诱导细胞大量凋亡,促使细胞生长停滞于G2/M期;此外,多甲氧基黄酮剂量依赖性的抑制胶质瘤细胞的侵袭。结论:PMFs能剂量和时间依赖性降低人胶质母细胞瘤细胞系U251的,同时显著诱导细胞瘤的凋亡和侵袭能力,提示PMFs可能对神经胶质瘤的高增殖性和侵袭性有一定的抑制作用,因此可能具有成为治疗神经胶质瘤药物的潜在应用价值。Objective： A broad spectrum of biological activities ofpolymethoxylated flavonoids, such as anti-oxidation, anti-cancer, anti-inflammation and anti-atherosclerosis, have aroused wide concern of investigators of at home and abroad. However, its potential role in human glioma treatment has not been extensively investigated. This study was aimed to investigate the effect of PMFs on the biological behavior of human glioblastoma cells to further study the potential role of PMFs in the treatment of human glioma. Methods： Human glioblastoma cells U251 were co-cultured with different concentration of PMFs （0,20,40,60,80, 100 ixg/mL）, and cell apoptosis was detected by Annexin V/PI staining, cell viability and invasion were detected by MTT methods and transwell invasion assay, respectively. Results： Following treatment with the increased dose and times, the proliferation of U251 cells was obviously attenuated. In addition, PMFs significantly augmented cell apoptosis in a dose-dependent manner, as well as cell cycle arrest at G2/M. Furthermore, treatment with higher dose of PMFs strikingly dampened cell invasion. Conclusion： PMFs may be of important role in the treatment of human glioma through inducing the apoptosis of U251 as well as inhibit the proliferation and invasion the cells significantly.

关 键 词：胶质瘤 多甲氧基黄酮 凋亡 增殖 侵袭 

分 类 号：R730.5[医药卫生—肿瘤]