血必净注射液对家兔左心缺血再灌注损伤肺脏的保护作用  

Protective Effect of Xuebijing injection on Acute Lung Injury Induced by Left Heart Ischemic Reperfusion in Rabbits

作　　者：王煜霞[1] 王雷[2] 姬明丽[1] 

机构地区：[1]新乡医学院病理生理学教研室,河南新乡453003 [2]新乡医学院第一附属医院普外二科,河南新乡453003

出　　处：《河南师范大学学报（自然科学版）》2013年第6期127-129,共3页Journal of Henan Normal University(Natural Science Edition)

基　　金：河南省高等学校青年骨干教师自主计划(2011GGJS-127);河南省教育厅自然科学研究计划项目(12B310017)

摘　　要：目的:探讨血必净注射液对家兔左心缺血再灌注损伤肺脏的保护作用.方法:60只健康成年家兔均分为两组:缺血再灌注组和血必净治疗组,两组均在缺血前30min(T1)、缺血30min(T2)、再灌注30min(T3)取颈外静脉血及颈总动脉血5ml;取支气管肺泡灌洗液5ml,检测外周血和支气管肺泡灌洗液中TNF-α水平及家兔肺组织TNF-α和ICAM-1表达情况.结果:1.血必净注射液提高缺血再灌注家兔PaO2;2血必净注射液降低缺血再灌注家兔外周血及支气管肺泡灌洗液TNF-α水平,降低肺组织TNF-α和ICAM-1表达.结论:血必净注射液通过降低左心缺血再灌注家兔外周血及支气管肺泡灌洗液中TNF-α含量及肺组织中TNF-α和ICAM-1的表达保护肺组织.Objective：Investigating the protective mechanisms of Xuebijing injection on acute lung injury induced by left heart ischemic reperfusion in rabbits . Metheds： Sixty rats were randomly divided into two groups： ischemic reperfusion group and Xuebijing injection treatment group. 5 ml blood samples from external jugular vein and carotid artery were collected in two groups at three point= thirty minutes before ligaturing（T1）, thirty minutes after ligaturing （T2） and thirty minutes after reperfusion （Ta）, 5 ml bronchialalveolar lavage fluid （BALF） was collected and determined. The expression levels of tumor necrosis factor-α（TNF-α）and intercellular adhesion molecule-1 （ICAM-1） in lung tissue were measured. Results= 1. Xuebijing injection improved the arterial partial pressure of oxygen. 2. Xuebijing injection reduced the level of TNF-α both in peripheral blood and in BALF and decreased the expression of TNF-α and ICAM-1 of lungs. Conclusion： Xuebijing injection protects lung tissues induced by left heart ischemic reperfusion in rabbits through reducing the level of TNF-α both in peripheral blood and in BALF, and inhibiting the expression of TNF-α and ICAM-1 of lungs.

关 键 词：血必净注射液 缺血再灌注损伤 肺脏 TNF-Α ICAM-1 

分 类 号：R363.2[医药卫生—病理学]