污染场地河道底泥浸出液对斑马鱼胚胎的毒性效应  

The Toxic Effects of Leachate of River Sediment in a Contaminated Site on Zebrafish (Brachydanio rerio) Embryos

作　　者：周艳[1,2,3] 徐建[1,2] 冯艳红[1,2] 张胜田[1,2] 丁浩然[1,2,3] 林玉锁[1,2] 

机构地区：[1]环境保护部南京环境科学研究所,南京210042 [2]国家环境保护土壤环境管理与污染控制重点实验室,南京210042 [3]南京农业大学资源与环境科学学院,南京210095

出　　处：《生态毒理学报》2013年第6期963-971,共9页Asian Journal of Ecotoxicology

基　　金：环保行业公益性科研专项(No.201009015);国家高技术研究发展计划(863计划)项目(No.2009AA063103);环保行业公益性科研专项(No.200909075)

摘　　要：为了评价某污染场地河道底泥对水生生物的毒性效应及其潜在生态风险,以斑马鱼(Brachydanio rerio)为受试生物,进行了河道底泥浸出液对斑马鱼胚胎的发育毒性实验。结果表明,5种底泥浸出液(A1、A2、A3、A4、A5)对斑马鱼胚胎的发育均有毒性效应,浸出液对斑马鱼胚胎的死亡率存在一定的剂量-效应关系,随着浸出液体积分数的增加,死亡率显著上升。浸出液对胚胎孵化抑制作用明显,随着浸出液体积分数的增加,孵化率显著下降。且能引起斑马鱼胚胎多种中毒症状,如水肿,鱼体弯曲,发育不全等异常现象,表明该段河道底泥存在潜在的生态风险。因此,在后期的污染场地河道整治中,需对河道底泥进行挖掘清理和无害化处理。To evaluate the potential ecological risk and the toxic effect of river sediment from a contaminated site on aquatic animals, zebrafish （Brachydanio redo） was chosen as the model animal and was exposed to the leachate of the sediment in the embryonic toxicity test. The toxic effects of five different leachate （A1 ~A2,A3, A4~A5） to the zebrafish embryos was studied. The results indicated that all the five leachate exhibited toxic effects on embryos by restraining the development of zebrafish embryos. Exposure to the leachate increased the mortality rate in a concentration - dependent way. A significant increase in mortality rate was observed, under elevated concentration. Furthermore, for all five leachate, a significant reduction in hatching rate was al- so recorded as the concentration of leachate increased. In addition, different abnormalities and toxic symptoms caused by the leachate were observed, including edema, fish curvature and immaturity. The results indicated potential ecological risk of the fiver sediment to the surrounding ecological environment, accordingly, the river sediment should be dredged and treated properly.

关 键 词：污染场地 河道底泥 斑马鱼胚胎 生物毒性 

分 类 号：X171.5[环境科学与工程—环境科学]