Sphingobacterium bambusaue及其紫外诱变菌株的石油降解功能  

Petroleum degradation capacity of Sphingobacterium bambusaue and its UV-mutants

作　　者：段盛文[1,2] 刘正初[1,2] 郑科[1,2] 冯湘沅[1,2] 成莉凤[1,2] 郑霞[1,2] 

机构地区：[1]中国农业科学院麻类研究所,湖南长沙410205 [2]农业部麻类生物学与加工重点实验室,湖南长沙410205

出　　处：《微生物学通报》2013年第12期2336-2341,共6页Microbiology China

基　　金：国家863计划项目(No.2012AA022209D);国家麻类产业技术体系建设专项项目(No.CARS-19-E24);湖南省自然科学基金项目(No.11JJ4021);国家微生物资源平台专项经费项目(No.NIMR2012-01-03)

摘　　要：【目的】研究Sphingobacterium bambusaue及其紫外诱变菌株的石油降解功能。【方法】紫外诱变后筛选石油降解高效菌株;以不同石油浓度、pH值及盐浓度优化培养条件,用重量法检测高效菌株石油降解率。【结果】发现菌株S.bambusaue在石油降解培养基中培养5 d的石油降解率为25.86%,UV诱变高效菌株IBFC2009-S3培养5 d的石油降解为42.85%,比始发菌株提高65.7%;UV诱变菌株IBFC2009-S3的优化培养条件为石油浓度0.5 g/L、pH值7.0以及NaCl质量浓度为10 g/L,其石油降解率可达50.51%。【结论】首次报道S.bambusaue具有石油降解功能;紫外诱变获得的菌株S3的石油降解能力较强。[Objective] Crude oil degradation by Sphingobacterium bambusaue and its UV mutants were studied. [Methods] The efficient crude oil degrading strains were obtained by UV mutagenesis, and the crude oil degradation rate was tested by gravimetric method in the culture conditions of different crude oil concentration, pH and salt concentration. ]Results] The crude oil degradation rate of the strain Sphingobacterium bambusaue and UV mutant IBFC2009-S3 cultured in crude oil medium for 5 d was 25.86% and 42.85% respectively, and the crude oil degradation rate of UV mutant IBFC2009-S3 increased 65.7% than that of the originating strains; the crude oil degradation rate of the strain IBFC2009-$3 was 50.51% in the optimum condition which was 0.5 g/L crude oil, pH 7.0, and 10 g/L NaC1. ]Conclusion] Crude oil degradation by Sphingobacterium bambusaue and its UV mutants IBFC2009-S3 have the higher ability of crude oil degradation.

关 键 词：Sphingobacterium bambusaue 紫外诱变菌株 石油降解 

分 类 号：X172[环境科学与工程—环境科学]