正常涎腺组织中E-cadherin，p16，RASSF1 A，DAPK和MGMT基因甲基化检测  

Methylation of E-cadherin,p16,RASSF1A,DAPK and MGMT in salivary gland tissues

作　　者：张春叶[1] 邓旭霞[2] 周美娟[3] 余宏男[2] 李蕾[1] 傅涵冰[1] 李江[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院口腔病理科,上海市口腔医学重点实验室,上海200011 [2]中国石油中心医院口腔科,河北廊坊065300 [3]河北省廊坊市人民医院口腔科,河北廊坊065300

出　　处：《军事医学》2013年第11期839-842,共4页Military Medical Sciences

基　　金：国家自然科学基金资助项目(30572054,81302360)

摘　　要：目的检测组织学正常的涎腺组织中5个抑癌基因的甲基化情况,为进行涎腺肿瘤的甲基化研究提供参照。方法甲基化特异性PCR(methylation-specific polymerase chain reaction,MSP)法分析60例组织学正常的涎腺组织中E-钙黏蛋白(cadherin),p16,RASSF1A,DAPK和MGMT基因启动子区的甲基化水平,并与前期研究中涎腺腺样囊性癌中的甲基化水平相比较,同时分析正常涎腺组织中E-cadherin(E-cad),p16,RASSF1A,DAPK和MGMT基因的甲基化与患者的性别、年龄及吸烟之间的关系。结果 13%(8/60)涎腺组织中发现存在甲基化,包括7%(4/60)E-cad,4%(2/60)p16,4%(2/60)RASSF1A,4%(2/60)DAPK,2%(1/60)MGMT。与之前腺样囊性癌中的结果比较,E-cad(P<0.01),p16(P<0.01),RASSF1A(P<0.01),DAPK(P<0.01)基因的甲基化在肿瘤组织及腺体组织中有明显差异。但涎腺组织中E-cad,p16,RASSF1A,DAPK和MGMT基因的甲基化与患者的性别、年龄及吸烟均无明显的相关性。结论正常的涎腺组织中E-cad,p16,RASSF1A,DAPK和MGMT基因的甲基化均为少发事件。Objective To investigate the methylation status of E-cadherin（E-cad）, p16, RASSF1A, DAPK and MGMT in histologically normal salivary gland tissues and provide reference for determination of the methylation status of salivary gland tumors.Methods Methylation of E-cad, p16, RASSF1A,DAPK and MGMT was analyzed using methylation-specific polymerase chain reaction （ MSP） .The results were compared with the methylation status of these genes in salivary adenoid cystic carcinoma （ ACC） tumor tissues in our previous studies and the association between promoter methylation of E-cad, p16, RASSF1A, DAPK, and MGMT on one hand and the patients′gender, age, smoking and types of gland on the other hand was also analyzed .Results Promoter methylation was detected in 8 of the 60 （13%） salivary glands, E-cad in 4（7%）, p16 in 2（4%）, RASSF1A in 2（4%）, DAPK in 2 （4%）, and MGMT in 1（2%）.Compared with our previous results, there was a significantly lower methylation ratio in promoter methylation of E-cad（P〈0.01）, p16 （P〈0.01）, RASSF1A （P〈0.01）,and DAPK （P〈0.01） in salivary gland tissues than in ACC tumor tissues.Conclusion Promoter methylation of E-cad, p16 and RASSF1A is a rare event in histologically normal salivary gland tissues .

关 键 词：涎腺 DNA甲基化 E-钙黏蛋白 基因 p16 RASSF1A DAPK 

分 类 号：R739.87[医药卫生—肿瘤]