甲状腺乳头状癌中Kiss-1、Ki-67、VEGF-C的表达及其定量相关性研究  

Expression of Kiss-1,Ki-67 and VEGF-C in papillary thyroid carcinoma and its stereological determination

作　　者：赵会丽[1] 赵琳[2] 王少霞[3] 贾贺堂[2] 

机构地区：[1]河北北方学院,河北张家口075000 [2]解放军309医院,北京100091 [3]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《军事医学》2013年第11期850-853,共4页Military Medical Sciences

摘　　要：目的探讨Kiss-1、Ki-67和VEGF-C在甲状腺乳头状癌(papillary thyroid carcinoma,PTC)和甲状腺滤泡性腺瘤(follicular adenoma,FA)中的表达及意义。方法采用免疫组织化学染色方法和显微图像定量分析技术测定44例PTC及12例FA石蜡包埋组织中Kiss-1、Ki-67及VEGF-C的表达。结果 PTC组Kiss-1和VEGF-C的积分光密度分别为475.56±126.02和805.29±226.05,Ki-67蛋白的增殖指数为(3.36±1.11)%,与FA组相比差异显著(P<0.05)。有淋巴结转移的PTC组Kiss-1和VEGF-C的积分光密度分别为408.12±124.05和912.63±108.12,Ki-67蛋白的增殖指数为(3.93±0.92)%,与无淋巴结转移的PTC组相比差异显著(P<0.05)。有被膜浸润的PTC组Kiss-1和VEGF-C的积分光密度分别为425.58±87.38和891.37±149.36,Ki-67蛋白的增殖指数为(3.79±1.09)%,与无被包膜浸润的PTC组相比差异显著(P<0.05)。线性相关分析显示,Ki-67与VEGF-C在PTC和FA组的表达呈正相关(P<0.05);Kiss-1与Ki-67、VEGF-C在PTC和FA组的表达呈负相关(P<0.05)。结论Kiss-1、Ki-67和VEGF-C有助于PTC与FA的鉴别诊断,并可作为PTC患者预后判断的指标。Objective To explore the expression and significance of Kiss-1, Ki-67 and VEGF-C in papillary thyroid carcinoma（PTC） and thyroid follicular adenoma （FA）.Methods Forty-four cases of PTC and twelve cases of FA paraffin-embedded tissues were used .Immunohistochemical staining and microscopic image analysis technique were used to analyze the expression of Kiss-1, Ki-67 and VEGF-C.Results The integrated optical density （IOD） of Kiss-1, and VEGF-C in the PTC groups was 475.56 ±126.02 and 805.29 ±226.05,respectively.The proliferation index of Ki-67 protein was （3.36 ±1.11） %and the difference between the PTC and FA groups was statistically significant （P〈0.05）.The IOD of the above two indices was 408.12 ±124.05 and 912.63 ±108.12 in the PTC with lymph node metastasis group , respectively, while the proliferation index of Ki -67 protein was （3.93 ±0.92） % and the difference vs the group without lymph node metastasis was significant （ P 〈0.05 ） .In the PTC with capsular infiltration group the IOD of above two was 425.58 ±87.38 and 891.37 ±149.36, the proliferation index of Ki -67 protein was （3.79 ±1.09） %and the difference with PTC group without capsular infiltrtion was statistically significant （P〈0.05）.Linear correlation analysis showed that Ki-67 and VEGF-C were with positively correlated in PTC and FA tissues （P〈0.05）,while Kiss-1 and Ki-67, VEGF-C were with negatively correlated in PTC and FA tissues （P〈0.05）.Conclusion Kiss-1, Ki-67 and VEGF-C can facilitate the differential diagnosis of PTC and FA , serving as prognostic indicators in patients with PTC .

关 键 词：甲状腺乳头状癌 甲状腺滤泡性腺瘤 KISS-1 KI-67 VEGF-C 免疫组织化学 显微图像定量分析 

分 类 号：R736.1[医药卫生—肿瘤]