穿心莲内酯通过Caspase-3/PARP途径诱导人舌癌Tb细胞凋亡作用研究  

Andrographolide induces Tb cell apoptosis by activating Caspase-3 /PARP

作　　者：亓翠玲[1] 周鑫磊[1] 叶杰[1] 杨扬[1] 章倩倩[1] 李江超[1] 王丽京[1] 

机构地区：[1]广东药学院血管生物学研究所,广州510006

出　　处：《中国药科大学学报》2013年第6期559-562,共4页Journal of China Pharmaceutical University

基　　金：国家自然科学基金资助项目(No.31271455)~~

摘　　要：研究穿心莲内酯对舌癌细胞(Tb细胞)凋亡的作用及其机制。用瑞士吉姆萨染色法、Hoechst 33258荧光染色法、原位Annexin V/PI双染和流式细胞术检测穿心莲内酯对Tb细胞凋亡的作用;Western blot检测穿心莲内酯诱导Tb细胞凋亡的机制。结果显示:穿心莲内酯处理Tb细胞后,与对照组相比,细胞凋亡数明显增加(P<0.05)。Western blot实验结果发现,穿心莲内酯处理组与对照组相比,聚腺苷酸二磷酸核糖转移酶(polyADP-ribosepolymerase,PARP)表达明显增强(P<0.05),表明穿心莲内酯可能通过活化PARP促进Tb细胞的凋亡。Andrographolide （Andro） is one of the major components extracted from Andrographis paniculata. As a natural plant medicine, Andro has been used to treat inflammation for thousands of years in Asia and South America. It has been reported to exhibit an important effect on tumor growth, yet its inhibition of Tb cell apoptosis has never been reported. To obtain the effect of Andro on Tb cell apoptosis, Swiss Giemsa staining, Hoechst 33258 dying, in situ Annexin V/PI double staining and FCM were used. Furthermore, its mechanisms of action on Tb cell apoptosis by Western blot were detected. It was found that Tb cell apoptosis rate and PARP expression level significantly increased after Andro treatment. Moreover, Andro promoted the Tb cell apoptosis through activating Casnase-3/PARP.

关 键 词：穿心莲内酯 细胞凋亡 PARP Tb细胞 

分 类 号：R285.5[医药卫生—中药学]