大肠杆菌内源过氧化氢检测方法的建立  

Detection Methods of Endogenous Hydrogen Peroxide in Escherichia coli

作　　者：王娜[1] 李欣[1] 朱文学[1] 刘严[1] 罗磊[1] 刘源[1] 

机构地区：[1]河南科技大学食品与生物工程学院,河南洛阳471023

出　　处：《农产品加工（创新版）（中）》2013年第12期67-70,共4页Farm Products Processing

基　　金：国家自然科学基金项目(31000017;30170238;30670070);国家重点实验室开放基金课题(SKL2011OP06)

摘　　要：通过比较分析找出一种快速检测大肠杆菌内源过氧化氢的方法。以大肠杆菌野生型及过氧化氢突变体为研究对象,采用辣根过氧化物酶-酚红法、荧光法、硫酸钛法3种方法检测大肠杆菌在同一生长时期内源过氧化氢的水平及其差异。3种检测方法绘制出来的标准曲线的线性相关系数分别是0.998 1,0.996 8,0.997 7,辣根过氧化物酶-酚红法和荧光法的结果显示,不同菌株的过氧化氢水平均为1～10μmol/L,释放量有明显的差异;硫酸钛法的检测区间为10～500μmol/L,明显检测不到大肠杆菌内源过氧化氢的释放量。结果表明,辣根过氧化物酶-酚红法操作比较方便;荧光法的灵敏度比较高,操作也比较简单方便,是一种快速检测大肠杆菌内源过氧化氢的理想方法。Discover a fast detection method of endogenous hydrogen peroxide in Escherichia coli through the analysis contrast. This experiment takes the Escherichia coli wild types and hydrogen peroxide mutants as the objects of study, through the horse radish peroxide enzyme-phenol red method, the fluorescence method, the titanium sulfate method three different methods detection of Escherichia coli in the same growth period of endogenous hydrogen peroxide level and differences. The standard curves using the three detection methods, whose respectively linear correlation coefficients are 0.998 1, 0.996 8, 0.997 7, hydrogen peroxide release quantity of different strains have the obvious difference according to the horse radish peroxide enzyme-phenol red method and the fluorescence method, scope between 1 μmol/L and 10 μmol/L. The detection sector between 10 μmol/L and 500 μmol/L through titanium sulfate method does not detection the release quantity of endogenous hydrogen peroxide in Escherichia coli. It shows that the horse radish peroxide enzyme-phenol red method is simple operation, and the fluorescence method is a fast detection method of endogenous hydrogen peroxide in Escherichia coli because the sensitivity is quite high and the operation is also simple and convenient.

关 键 词：大肠杆菌 内源过氧化氢 突变株 检测方法 

分 类 号：R378.21[医药卫生—病原生物学]