肺癌浸润淋巴细胞的分离及增殖研究  

Experimental research of tumor infiltrating lymphocytes for treatment of lung cancer

作　　者：张毅[1] 赖百塘[2] 李世业[1] 汪蕙[2] 湛秀萍[2] 刘桂枝[2] 王月[2] 

机构地区：[1]北京市结核病胸部肿瘤研究所医院胸外科,101149 [2]北京市结核病胸部肿瘤研究所医院细胞生物室,101149

出　　处：《中国肺癌杂志》2000年第6期441-444,共4页Chinese Journal of Lung Cancer

摘　　要：目的　从肺癌患者手术切除的肿瘤组织中分离出肺癌浸润淋巴细胞 (TIL) ,经rIL 2体外激活培养 ,研究TIL体外分离、增殖的条件和特点。方法　应用机械、酶消化和不连续密度梯度离心法从 67例肺癌患者手术切除的实体瘤中分离得到TIL ,其中 3 4例TIL放入 10 %人AB血清RPMI 164 0液中培养。结果　本组 67例标本中 ,每克瘤组织获得的TIL平均为 6.84× 10 6个。在培养的 3 4例TIL中 ,第 7～ 2 8天之间增殖最旺盛 ,以后增殖活性降低 ,数量减少。增殖达到 10 9以上有 17例 ,可回输率为 5 0 %。Ⅰ期患者分离获取TIL数明显多于Ⅲ、Ⅳ期 ,但在增殖倍数上无显著性差异 ;鳞癌与腺癌分离获取TIL数无显著性差异 ,但腺癌TIL扩增明显比鳞癌好。结论　TIL能有效地分离和增殖 ,并已具备了应用于临床治疗肺癌的条件。Objective To explore the conditions and characterization of tumor infiltrating lymphocytes(TILs) isolation and expansion. Methods TILs were separated from tissue samples which obtained from the surgically removed tumor of 67 patients with lung cancer by mechanic and enzyme digestion and discontinuous density Ficoll paque gradient centrifugation ways. TILs from 34 patients were incubated with 10% human type AB serum RPMI 1640 in vitro. Results The average number of TIL was 6.84×10 6 per gram tumor tissue. The number of TIL from patients in stage Ⅰ was greater than that from patients in stage Ⅲ and Ⅳ, but with no difference in expanding among them. The number of TIL from patients with adenocarcinoma was similar to that with squamous cell carcinoma, but TIL isolated from adenocarcinoma expanded much more faster than that from squamous cell carcinoma. TIL expanded rapidly during 7 28 days and most of them appeared the maximum expansion within 35 days. TIL incubated from 17 of 34 patients had expanded exceeding 1×10 9, so the rate of infusion was 50%. Conclusion The results suggest TIL can be effectively separated from the tumor and expanded in vitro and these provide the basis for adoptive immunotherapy with TIL in the treatment of lung cancer.

关 键 词：肺肿瘤 肿瘤浸润淋巴细胞 分离 增殖 

分 类 号：R734.2[医药卫生—肿瘤]