多发性骨髓瘤中p15^(INK4B)和p16^(INK4A)基因高甲基化的检测  

Detection of High Methylation of p15^(INK4B) and p16^(INK4A) Genes in Multiple Myeloma

作　　者：范洪涛[1] 郭秀枝[2] 吴穷[2] 周涛[2] 谭广销[2] 王绿娅[3] 张学莉[4] 罗更新[4] 许敏华[4] 

机构地区：[1]霍华德大学 [2]暨南大学医学院血液病研究室,广州510632 [3]北京安真医院,北京100029 [4]暨南大学医学院第一附属医院血液科,广州510632

出　　处：《中国实验血液学杂志》2000年第4期271-274,共4页Journal of Experimental Hematology

摘　　要：为探索p15 INK4B和p16INK4A基因在多发性骨髓瘤中的甲基化的表达 ,采用敏感的甲基化特异的MSP PCR测定方法 ,检测了 2 4例多发性骨髓瘤 (MM ) p15 INK4B和p16INK4A基因甲基化的情况。结果显示 ,MM中 p15 INK4B和 p16INK4A基因甲基化发生率分别为 70 .8% (17/ 2 4 )和 5 8.3% (14/2 4 ) ,产物分别为 148bp和 15 0bp的片段 ,其中 2例Ⅱ期和 2例Ⅲ期的有浆细胞瘤的MM患者发生 2个基因同时甲基化 ,有 5例 2个基因都没有发生甲基化。这 5例的瘤细胞均为分化较成熟的小浆细胞。结论提示 ,p15 INK4B和 p16INK4A基因甲基化在MM细胞中有一定的发生率 。To study the action, characteristics and expression of high methylation of p15 INK4B and p16 INK4A genes in multiple myeloma(MM), the sensitive methylation specific PCR method was employed to detect the hypermethylation of p15 INK4B and p16 INK4A in 24 patients with MM. Results showed that the methylation incidence of p15 INK4B and p16 INK4A genes were 70.8%(17/24) and 58.3%(14/24) in the MM patients, with the products of 148 bp and 150 bp fragments, respectively. The methylation of p15 INK4B and p16 INK4A genes were simultaneously happened in MM patients of plasmocytoma type with two cases at Ⅱ phase and two cases at Ⅲ phase. The simultaneous non-methylation of p15 INK4B and p16 INK4A genes were founded in five cases of MM patients, all of the tumor cells were of small plasmocyte type with mature differention. Conclusion suggested that there were high incidence of methylation of p15 INK4B and p16 INK4A genes in patients with MM. Hypermethylation can be detected in the early stage of disease, which was associated with its progress. It indicated a bad prognosis when methylation happended simultaneously in the two genes. Methylation of p15 INK4B and p16 INK4A genes may be related to the pathogeny of MM.

关 键 词：多发性骨髓瘤 甲基化 P15^INK4B 基因 P16^INK4A 

分 类 号：R733.3[医药卫生—肿瘤]