潮湿纤维单胞菌和绿色木霉混合接种对不同食用菌菌渣相关酶活性的影响  

Study on the Related Enzyme Activities in the Residues of Different Mushroom Substrates Inoculated with Cellulomonas uda and Trichoderma viride

作　　者：谢小林[1,2] 顾振红[2] 陈美标[1] 姚青[2] 朱红惠[1] 

机构地区：[1]广东省微生物研究所广东省菌种保藏与应用重点实验室广东省微生物应用新技术公共实验室省部共建华南应用微生物国家重点实验室,广州510070 [2]华南农业大学园艺学院,广州510642

出　　处：《生物技术通报》2013年第12期162-166,共5页Biotechnology Bulletin

基　　金：国家科技支撑计划项目(2012BAD14B16);广东省科技攻关项目(2008B021000047);广东省-中科院产学研项目(2010B090300067);广东省省部产学研结合项目(2011B090400613)

摘　　要：利用产酶试验和滤纸崩解试验确定纤维降解细菌(潮湿纤维单胞菌(Cellomonas uda))和真菌(绿色木霉(Trichoderma viride))单一菌株酶活性和滤纸崩解能力,依据滤纸崩解度和羧甲基纤维素酶(CMCase)活性进行不同混合接种比例降解不同食用菌菌渣,探讨不同菌渣发酵过程中细菌和真菌的最适配比比例和相关酶活性变化。试验结果表明,Cellulomonas uda在产酶和崩解培养基中CMCase活性均要高于Trichoderma viride,混合菌群发酵极大地提高了酶活性;以桉木屑-秀珍菇菌渣、杂木屑-灵芝菌渣和杂木屑-秀珍菇菌渣为唯一碳源发酵4 d后,其CMCase活性最大值分别为2 809、3 606和971 U/mL,滤纸酶(FPase)活性最大值分别为486、454和151 U/mL。为后续大规模固体发酵提供依据,桉木屑-秀珍菇菌渣、杂木屑-灵芝菌渣和杂木屑-秀珍菇菌渣分别采用潮湿纤维单胞菌和绿色木霉配比为1∶3、3∶1和1∶2为宜。Enzyme production test and collapse of filter paper test were used to establish the cellulase activity and the collapse degree of filter paper with single strain of cellulolytic bacterium（Cellulomonas uda）and fungus（Trichoderma viride）. Then mixing ratios of cellulolytic bacterium and fungus were determined to degrade the residues of different mushroom substrates in the liquid fermentation on the basis of the collapse degree of filter paper and carboxymethyl cellulase（CMCase）activity, to study on the optimal mixing ratio of cellulolytic bacterium and fungus and change of related enzyme activities in the fermentation of residues of mushroom substrates. The results showed that the CMCase activity of Cellulomonas uda was higher than those of Trichoderma viride in both the cellulase production medium and collapse of filter paper medium. However, the mixed fermentation of Cellulomonas uda and Trichoderma viride greatly improved the cellulase enzyme activity. After four days liquid fermentation, using eucalypt sawdust-pleurotus geesteranus, mixed sawdust-ganoderma and sawdust-pleurotus geesteranus as only carbon source, the maximum CMCase activity was 2 809, 3 606 and 971 U/mL and the maximum filter paper（FPase）activity was 486, 454 and 151 U/mL, respectively. These results can provide basis for the large scale solid state fermentation, and the suitable mixing ratio of Cellulomonas uda and Trichoderma viride for eucalypt sawdust-pleurotus geesteranus, mix sawdust-ganoderma and mixed sawdust-pleurotus geesteranus was 1∶ 3, 3∶ 1 and 1∶ 2, respectively.

关 键 词：潮湿纤维单胞菌 绿色木霉 混合比例 纤维素酶 食用菌菌渣 

分 类 号：S646[农业科学—蔬菜学]