乌贼墨多肽体外抗氧化活性研究  

Study on the Antioxidant Activity of Sepia Ink Peptides in vitro

作　　者：景奕文[1] 杨最素[1] 丁国芳[1] 黄芳芳[1] 

机构地区：[1]浙江海洋学院食品与医药学院,浙江省海洋生物医用制品重点工程技术研究中心,浙江舟山316004

出　　处：《安徽农业科学》2013年第27期10971-10973,11025,共4页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金面上项目(81273429);浙江省科技厅重大专项(2010C13009;2011C02003);浙江省自然科学基金立项(LY12C20005;LY12C20008);舟山市科技计划项目(2012C23023)

摘　　要：[目的]研究乌贼墨多肽体外抗氧化活性。[方法]采用分光光度法测定多肽对2,2’-连氮基-双-(3-乙基苯并二氢噻唑林-6-磺酸)二铵盐(ABTS)、1,1-二苯基-2-三硝基苯肼(DPPH)、羟自由基(·OH)的清除能力和还原能力,利用琼脂糖凝胶电泳检测多肽对羟自由基诱导的DNA损伤的保护作用。[结果]乌贼墨多肽具有明显清除ABTS自由基(半数有效浓度EC50为3.45 mg/ml)、·DPPH自由基(EC50为4.34 mg/ml)和羟自由基(EC50为3.30 mg/ml)的能力;当乌贼墨多肽的浓度达到5.00 mg/ml时,还原力达到0.387。[结论]乌贼墨多肽具有较好的抗氧化活性。[ Objective ] To study the antioxidant activity of sepia ink peptides in vitro. [ Method] The spectrophotometry method was adopted to determine the scavenging and reducing capacity of polypeptide on ABTS, 1,1-diphenyl-2-picrylhydrazy] （ · DPPH） and hydroxyl radical; the protective effect of the peptides against hydroxyl-radical-induced DNA damage was measured by DNA agarose electrophoresis. [ Result ] The results showed that the ABTS, DPPH and hydroxyl free radicals radical-scavenging activity were increased with concentrations of the pep- tides, and the ECso values were 3.45 mg/ml, 4.34 mg/ml, 3.30 mg/ml, respectively. [ Conclusion ] The peptides isolated from sepia ink hydrolyzate exhibited an obvious capability of antioxidation.

关 键 词：乌贼墨 多肽 抗氧化 

分 类 号：S567[农业科学—中草药栽培]