3株大型真菌最佳培养条件的筛选研究  

Selection of Optimum Culture Conditions for Three Macrofungal Strains

作　　者：李丹[1] 戴月婷[1] 李玉[1] 

机构地区：[1]吉林农业大学食药用菌教育部工程研究中心,吉林长春130118

出　　处：《安徽农业科学》2013年第28期11325-11325,11339,共2页Journal of Anhui Agricultural Sciences

基　　金：现代农业产业技术体系建设专项基金项目(CARS24);吉林省重大项目(10ZDGG003)

摘　　要：[目的]筛选辽宁省仙人洞国家级自然保护区的3种大型真菌———蛹虫草(Cordyceps militaris)、微细小菇(Mycena minutula)和头状马勃(Calvatia craniiformis)的最佳培养条件。[方法]首先进行子实体分离,并获得优势菌株,比较不同碳源、氮源、pH对蛹虫草、微细小菇和头状马勃菌丝生长速度及长势的影响。[结果]蛹虫草、微细小菇和头状马勃3个菌株的最适碳氮源、pH分别为淀粉、谷氨酸、pH5或6;淀粉、蛋白胨、pH 8;玉米粉、蛋白胨、pH 5。[结论]该研究可为充分利用野生菌物资源提供指导。[ Objective ] The aim was to screen the optimum culture conditions for three macrofungal strains from Xianrendong National Nature Reserve in Liaoning province--Cordyceps militaris, Mycena minutula and Calvatia craniiformis. [ Method ] Fruiting body was separated and ob- tained the dominant stains, then the effects of four different kinds of carbon sources, nitrogen sources and pH on the mycelial growth rate and growth vigor were compared. [ Result ] The best culture conditions of the 3 strains, Cordyceps militar/s, Mycena minutula and Calvatia craniiformis, were as follows ： Starch, glutamate, pH 5 or 6 ; starch, peptone, pH 8 ; corn meal, peptone, pH 5. [ Conclusion] The study provides a guidance for take full advantage of wild fungal resources.

关 键 词：蛹虫草 微细小菇 头状马勃 菌株 培养条件 

分 类 号：S646[农业科学—蔬菜学]