毛蚶正丁醇相抗卤虫化学成分分析  

Bioactivity Evaluation and Constituent Analysis of the nbutanol Fractions fromScapharca Subcrenata Lischke

作　　者：孙雪萍[1] 徐艳[1] 宋成芝[2] 童万平[1] 

机构地区：[1]广西海洋研究所海洋生物技术重点实验室,广西北海536000 [2]昭通师范高等专科学校化学系,云南昭通657000

出　　处：《广西科学》2013年第4期272-275,共4页Guangxi Sciences

基　　金：广西科学研究与技术开发计划项目(桂科攻11107011-8);广西科学研究与技术开发计划项目(桂科攻11107011-6)资助;广西科学院基本科研业务费项目(13YJ22HYS15)

摘　　要：应用抗卤虫(Artemia salina)生物活性筛选模型,分析南海毛蚶(Scapharca subcrenata)乙醇浸膏和正丁醇相的生物活性成分。从毛蚶(S.subcrenata)正丁醇萃取相中共分得4个化合物,包括3个甾体类化合物5-烯-胆甾醇(1),胆甾醇(2),3-酮-20-烯孕甾烷(3)和1个吲哚类化合物3-carboxy-indole(4),化合物(1)^(3)在50μg/mL时对卤虫的致死率分别为65.0%,60.0%和58.3%。The bioactivities of ethanolic extract and n-butanol fractions of Scapharca subcrenata from the South China Sea were evaluated by the model of lethality to brine shrimp Artemia salina.The ethanolic extract and n-butanol fractions showed lethality.The compounds in the n-butanol were separated,and four compounds were identified as cholest-5-en-3-ol （1）,Cholesterol （2）,5a-pregn-20-en-3-one （3） and 3-carboxy-indole （4）.The compounds 1～3 belong to sterols and their lethal rate was 65.0%,60.0% and 58.3% in 50μg/mL,respectively,suggesting that these steroids should be the bioactive substances of S.Subcrenata lischke.

关 键 词：毛蚶 甾体 生物活性 

分 类 号：P714.5[天文地球—海洋科学] R914[医药卫生—药物化学]