紫外-可见分光光度法测定不同产地山楂果实中总黄酮含量  

Analysis of Total Flavonoids in Crataegus Pinnatifida BgeFrom Different Areas by UV-VIS Spectrophotometry

作　　者：马燕斌[1] 曾建勋[1] 张俊鹏[1] 李瑞明[1] 刘怡[1] 梁耀荣[1] 

机构地区：[1]中山大学附属第一医院药学部,广东广州510080

出　　处：《中国药物经济学》2013年第6期24-26,共3页China Journal of Pharmaceutical Economics

基　　金：广东省重大科技专项项目(2011A080300004);广东省中医药局科研项目(20121145);广州市生物产业示范工程发展专项项目(2012-243)

摘　　要：目的建立紫外-可见分光光度法测定不同产地山楂果实中总黄酮含量。方法采用超声法提取山楂果实中的总黄酮,采用紫外-可见分光光度法测定不同产地山楂总黄酮含量,检测波长为500nm。结果芦丁在质量浓度20-120μg/mL（r=0.9993）范围内与吸光度值线性关系良好,平均回收率为100.1%,RSD=0.3%（n=6）,来自8个产地的总黄酮含量在3.53%-4.12%之间。结论本方法简便、准确,重复性好,可快速测定山楂果实中总黄酮的含量;不同产地山楂总黄酮的含量存在一定差异,本实验可为控制山楂的质量提供参考依据。Objective To establish a UV-VIS spectrophotometry method to determine total flavonoids in Crataegus pinnatifida Bge. from different areas. Methods Total flavonoids of Crataegus pinnatifida Bge. was extracted by ultrasonic method, and was determined by UV-VIS spectrophotometry at 500 nm. Results The linearity of rutin was20-120μg/mL（r=0.9993）, and the average recovery ratios was 100.1%. The RSD was 0.3%（n=6）. The contents of total flavonoids ranged from 3.53%to 4.12%. Conclusion The method was simple and accurate, and it could be used for the determination of total flavonoids quickly. The contents of total flavonoids was different from different areas.It provided some academic basis for quality standard of Crataegus pinnatifida Bge..

关 键 词：山楂 总黄酮 紫外-可见分光光度法 

分 类 号：R927.2[医药卫生—药学]