β-catenin和Wnt-1在食管癌组织中的表达及其临床意义的研究  

Expression of β-catenin and Wnt-1 in esophageal carcinoma and their clinical significances

作　　者：王丹[1] 喻春钊[2] 

机构地区：[1]南京医科大学第二附属医院ICU,江苏南京210011 [2]南京医科大学第二附属医院普通外科,江苏南京210011

出　　处：《中国医药导报》2013年第36期89-93,共5页China Medical Herald

基　　金：国家自然科学基金资助项目(编号81172269);国家自然科学基金资助项目(编号30972910);中国博士后科学基金项目(编号20060390294);江苏省基础研究计划(自然科学基金)-面上研究项目(编号BK2011858)

摘　　要：目的研究β-catenin和Wnt-1在食管癌组织中的蛋白表达水平及其与肿瘤病理参数和预后的关系。方法 2012全年选取江苏省人民医院病理科保存的40例手术切除食管癌蜡块标本和10例癌旁组织,将其分为实验组和对照组,应用免疫组化方法检测其β-catenin和Wnt-1的蛋白表达水平,采用JD-801病理图像分析系统,利用计算机技术对病灶影像定量分析其光密度参数、灰度分布。使用Stata 9.0(USA)软件包,参考值选用10例癌旁组织的蛋白表达水平,将肿瘤组织中的表达水平与参考值进行比较,分为高表达组和低表达组,分析两组生存率是否存在差别采用Kaplan-meier法。结果 Wnt-1的表达与食管癌预后的关系:高表达组生存率比低表达组高,差异有统计学意义(P=0.0177)。β-catenin的表达与食管癌预后的关系:高表达组生存率比低表达组高,但差异无统计学意义(P=0.1010)。结论 Wnt-1和β-catenin作为Wnt信号通路的相关蛋白,与食管癌发展、侵袭和预后等方面有密切关系。Objective To investigate the expression of β-catenin and Wnt-1 in esophageal carcinoma and their correlations with the pathologic parameter of cancer and prognosis of esophageal carcinoma. Methods This study was performed at the Pathological Department of Jiangsu People′s Hospital in 2012. 40 esophageal cancer sections and 10 surrounding tissues embedded in paraffin was enrolled. The β-catenin and Wnt-1 expression by immunohistochemistry were detected. Quantification was performed by measuring the intensity of the signals with JD-801 Pathological image analysis system. All the esophageal cancer sections were divided into the high expression group and low expression group. Statistical analysis of the β-catenin and Wnt-1 expression between the two groups were performed using Stata 9.0（USA）. Univariate Kaplan-Meier analysis was used to estimate the survival rate between the two groups. Results Wnt-1 expression was associated with a significantly higher survival rate by Kaplan-Meier analysis（P = 0.0177）. β-catenin high expression group had a high survival rate,while there was not statistically significant（P = 0.1010）. Conclusion As the correlated proteins of WNT signal pathway,the expression level of Wnt-1 and β-catenin are close relationship with progression,invasion and the prognosis of esophageal carcinoma.

关 键 词：食管癌 免疫组化 Β-CATENIN WNT-1 

分 类 号：R735.1[医药卫生—肿瘤]