高尔基体蛋白联合甲胎蛋白检测在原发性肝癌早期诊断中的价值  

Value of GP73 and AFP in early diagnosis of primary liver cancer

作　　者：徐向勇 刘显畅 李正峰[2] 祝成亮[3] 

机构地区：[1]武汉钢铁(集团)第二职工医院心内科,湖北武汉430085 [2]武汉市儿童医院,湖北武汉430080 [3]武汉大学人民医院检验科,湖北武汉430060

出　　处：《中国医药导报》2014年第1期83-85,共3页China Medical Herald

基　　金：国家自然科学基金项目(编号81101485)

摘　　要：目的探讨血清高尔基体蛋白73（GP73）、甲胎蛋白（AFP）联合检测对原发性肝癌（PHC）早期诊断中的作用。方法选取2007年7月～2012年7月武汉钢铁（集团）第二职工医院PHC患者76例（PHC组）、肝硬化患者53例（肝硬化组）和正常人群50例（对照组），采用酶偶联吸附法和化学发光免疫分析法进行血清GP73、AFP检测，分析两者单独及联合检测在早期诊断PHC中的价值。结果①PHC组患者血清GP73[（208．3±53．6）μg/L]AFP[（871．2±497．6）μg/g／L]明显高于肝硬化组[（74．8±26．4）、（91．7±68．5）μg／L]±11对照组[（31．6±7．7）、（12．7±7．9）μg/L]，差异均有统计学意义（P〈0．05）。②PHC组患者血清GP73和AFP阳性检出率[81．6％（62／76）、63．2％（48／76）1分别高于肝硬化组[15．1％（8／53）、17．0％（9／53）]和对照组[0．0％（0／50）、0．0％（0／50）]，差异均有统计学意义（P〈0．05）。③联合检测对PHC诊断灵敏度[96．1％（73／76）]高于GP73和AFP单项检测[81．6％（62／76）、63．2％（48／76）]，差异有统计学意义（P〈0．05）；而检测的特异性与单项检测相比差异无统计学意义（P〉0．05）。结论PHC患者血清中GP73和AFP水平较高．两者联合检测可以显著提高对PHC早期诊断的能力。Objective To explore the clinical value of combined detection of serum GP73, AFP in the early diagnosis of primary liver cancer （PHC）. Methods 76 cases of PHC, 53 cases of liver cirrhosis, 50 cases of normal people from July 2007 to July 2012 in the Second Worker＇s Hospital of WISCO were selected as PHC group, liver cirrhosis group and control group respectively, the enzyme coupling adsorption method and chemiluminesent lmmunoassay assay were used to detect the serum GP73, AFP, the value of single and combined detection in early diagnosis of PHC were analyzed. Results ①The serum GP73, AFP in PHC group [（208.3±53.6）, （871.2±497.6） μg/L] were higher than those in liver cirrhosis group [（74.8±26.4）, （91.7±68.5） μg/L] and control group [（31.6_±7.7）, （12.7_±7.9） I.Lg/L], the differences were statistically significant （P 〈 0.05）. ②Positive detection rate of serum GP73 and AFP in PHC group [81.6% （62/76）, 63.2% （48/76）] were higher than those in liver cirrhosis group [15.1% （8/53）, 17.0% （9/53）] and control group [0.0% （0/50）, 0.0% （0/50）], the differences were statistically significant （/9 〈 0.05）. ③PHC diagnosis sensitivity by combined detection [96.1%＇ （73/76）] was higher than that by GP73 and AFP single detection [81.6% （62/76）, 63.2% （48/76）], the differences were ＇statistically significant （P 〈 0.05）. The differences of PHC diagnosis specificity between combined detection and GP73 and AFP single detection was not statistically significant （P 〉 0.05）. Conclusion PHC patients have a higher GP73 and AFP levels, the combined detection can significantly improve the diagnosis of early PHC.

关 键 词：高尔基体蛋白 甲胎蛋白 原发性肝癌 诊断 

分 类 号：R735.7[医药卫生—肿瘤]