珍稀食药用真菌猴头菇寡聚糖的分离纯化及抗氧化活性的研究  

Extraction,purification and antioxidant activity of oligosaccharides from Hericium erinaceus

作　　者：王芳[1] 丁祥[1] 宋波[1] 王婷[1] 李健[1] 曾益春[1] 许婷[1] 钟洁[1] 侯万儒[1] 侯怡铃[1] 

机构地区：[1]西华师范大学生命科学学院、西南野生动植物资源保护教育部重点实验室,南充637002

出　　处：《四川大学学报（自然科学版）》2013年第6期1339-1346,共8页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金(31200012);四川省科技厅应用基础项目(2011JY0135);四川省科技厅应用基础项目(2013JY0094)

摘　　要：从猴头菇(Hericium erinaceus)子实体中,经过除杂、粉碎、热水浸提、醇沉处理后,得到了猴头菇杂聚糖粗提物,并运用DEAE-cellulose column进行柱层析分离纯化,得到猴头菇寡聚糖(HEO-A).通过水解,单糖分析,扫描电镜技术(SEM),高效凝胶渗透色谱(HPGPC),红外光谱技术(IR)和核磁共振技术(NMR)对猴头菇寡聚糖进行了初步的结构分析.结果显示,猴头菇寡聚糖由木糖和葡萄糖两种单糖构成,均重分子量为1877D.在体外化学模拟条件下,研究了不同浓度的猴头菇寡聚糖对羟基自由基(·OH)、1,1-二苯基-2-苦苯肼自由基(DPPH·)及ABTS自由基的清除作用.对DPPH·自由基的清除能力,其IC50值为12.5mg/mL;对·OH自由基的清除能力,当HEO-A浓度达到5mg/mL时,其清除率可达到最大(37.51%);对ABTS自由基的能力,其IC50值为0.93mg/mL,且当HEO-A的浓度为1.5mg/mL时,清除率即可达95.44%.这些结果说明猴头菇寡聚糖(HEO-A)具有显著的抗氧化功能,可以作为一种理想的天然抗氧化剂资源.A oligosaccharide was extracted from the fruiting bodies of Hericium erinaceus with water and then successively purified by DEAE-cellulose 52 column chromatography ,yielding one major oligosac-charide fractions ：HEO-A .Structural features of HEO-A were investigated by a combination of total hydrolysis ,high-performance gel permeation chromatography analysis （HPGPC） ,scanning electron mi-croscope （SEM） ,infrared （IR） spectra and nuclear magnetic resonance （NMR） spectroscopy studies . The results showed that the average molecular sizes of HEO-A were approximately 1877 D and was mainly composed of xylose and glucose .The antioxidant activity analyses revealed that HEO-A pos-sessed considerable antioxidant activity .The IC50 value of the DPPH · radical scavenging activity is 12 .5 mg/mL .The · OH radical scavenging ability reaches the maximum （37 .51% ） when the concentration of HEO-A reached 5mg/mL .The IC50 value of the ABTS radical scavenging activity is 0 .93 mg/mL , and w hen the concentration of HEO-A is only 1 .5 mg/mL ,removal rate is up to 95 .44% .Accordingly , Hericium erinaceus might serve as an effective antioxidative healthcare food and source of natural an-tioxidants .

关 键 词：猴头菇(Hericium erinaceus) 寡聚糖 分离纯化 抗氧化 

分 类 号：Q53[生物学—生物化学]