超高压液相色谱-串联质谱法(LC-MS-MS)测定鱼肉中磺胺类兽药残留的基质效应  

Matrix Effects in the Determination of Sulfonamide Residues in Fish Meats by Ultra Performance Lliquid Chromatography-Tandem Mass Spectrometry (LC-MS-MS)

作　　者：颜春荣[1] 张晓强[1] 林慧[1] 徐春祥[1] 

机构地区：[1]江苏省产品质量监督检验研究院,江苏南京210007

出　　处：《肉类研究》2013年第10期17-20,共4页Meat Research

基　　金：江苏省质量技术监督局科技项目(KJ122701)

摘　　要：采用提取后添加法从提取溶剂、净化方法、同位素内标、基质标曲等方面对超高压液相色谱-串联质谱法测定鱼肉中的磺胺类兽药残留进行基质效应的研究。结果表明:以乙腈为提取溶剂,经MCX固相萃取柱净化和液液萃取净化可以降低基质效应的影响,采用基质添加标准曲线和同位素内标可以对基质效应的影响进行补偿。Extracts spiked with sulfonamide standards were used as samples for evaluating matrix effects in the determination of veterinary sulfonamides residue in fish meats by ultra-high pressure liquid chromatography-tandem mass spectrometry （LC-MS-MS） with respect to extraction solvents, clean-up methods, isotope internal standard and standard calibration curve. Results suggested that the matrix interferences could be reduced by extraction with acetonitrile and clean- up on an MCX cartridge or by liquid-liquid extraction and compensated by a calibration curve for spiked samples and isotope internal standard.

关 键 词：液相色谱-串联质谱(LC-MS-MS) 鱼肉 磺胺类兽药 基质效应 

分 类 号：S859.84[农业科学—临床兽医学]