抗CD28+B7.1单抗共刺激PBLs诱导肝癌细胞凋亡的蛋白激酶信号转导  被引量：1

The signal transduction of protein kinase in PBLs co stimulated by anti CD28+B7 1 McAb and the induction of apoptosis of the hepatoma cells<eauthor><Author>YANG Hong 1, CHEN Geng fu 1, HUANG Yu shan 2, HUANG Shu lin 1 (1 Dep</em> <p class='article-op'> <span id="bdshare" class="bdshare_t bds_tools get-codes-bdshare baidufx"><span><span class="bds_more" style="display: block; width: 62px; height: 29px; background: url(/images/share.gif) no-repeat!important; padding: 0;"></span></span></span> </p> <p class="article-source"> <a href ='/article/read.aspx?id=4817938' class='btnTitle btnIsView' target='_blank'><img alt='在线阅读' src='/images/btn-read.png'/></a><a class='btnTitle btnIsView' href="javascript:showdown('C5AECHUcgH+dAJ5b3b5zwgK8aTFRYIvjLzmm/acED2o=')"><img alt="下载全文" src="/images/btn-down.png" /></a> <input id="hiddowninfo" type="hidden" value="C5AECHUcgH+dAJ5b3b5zwgK8aTFRYIvjLzmm/acED2o="/> </p> <div class="collect" style="display: none"> <h5>我的收藏</h5> <div class="cnt"> <p> 名 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class='type_ico_id'></b></a><a href='/zk/search.aspx?key=A%3d%5b%e9%bb%84%e7%8e%89%e7%8f%8a%5d' title='黄玉珊[2]'>黄玉珊[2]</a> <a href='/writer/article.aspx?id=100000008029171' target='_blank' ><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=A%3d%5b%e9%bb%84%e6%a0%91%e6%9e%97%5d' title='黄树林[1]'>黄树林[1]</a> <em> </em></p><p class='organ'><strong>机构地区：</strong><a href='/organ/article.aspx?id=132&subid=&showname=&searchtype=' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=S%3d%e5%b9%bf%e4%b8%9c%e8%8d%af%e5%ad%a6%e9%99%a2%e5%88%86%e5%ad%90%e7%94%9f%e7%89%a9%e5%ad%a6%e7%a0%94%e7%a9%b6%e5%ae%a4' title='[1]广东药学院分子生物学研究室,广东广州510224'>[1]广东药学院分子生物学研究室,广东广州510224</a> <a href='/organ/article.aspx?id=22210&subid=&showname=&searchtype=' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=S%3d%e4%b8%ad%e5%b1%b1%e5%a4%a7%e5%ad%a6%e7%94%9f%e5%91%bd%e7%a7%91%e5%ad%a6%e9%99%a2' title='[2]中山大学生命科学院,广东广州510275'>[2]中山大学生命科学院,广东广州510275</a> <em> </em></p><p class='from'><strong>出　　处：</strong><a href='/journal/summary.aspx?gch=90206X' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=J%3d%5b%e5%85%8d%e7%96%ab%e5%ad%a6%e6%9d%82%e5%bf%97%5d' title='免疫学杂志'>《免疫学杂志》</a>2001年第1期40-42,共3页<em>Immunological Journal</em></p><p class='fund'><strong>基　　金：</strong>国家自然科学基金!(396 40 0 0 0 5 );广东省科教兴医"五个一"工程重点课题!(粤卫科[1996 ]2 0号 )资助项目</p><p class='abstrack'><strong>摘　　要：</strong>目的研究 T淋巴细胞活化、增殖、介导肝癌细胞凋亡过程中其蛋白激酶 C(PKC)和酪氨酸蛋白激酶 (TPK)的活性变化。方法用抗 CD2 8+ B7.1(CD80 )单克隆抗体共刺激正常人外周血淋巴细胞 (PBL s)后作用于肝癌细胞 (BEL -740 2 )。结果激活的 T细胞中 PKC、TPK活性明显增强 ,并与肝癌细胞凋亡程度呈正相关。结论 PKC、TPK在 T细胞活化。<em>ObjectiveTo investigate the activities of protein kinase C (PKC) and tyrosine protein kinase (TPK) in the activated and proliferating T cells and the induction of apoptosis of the hepatoma cells MethodsHealthy human PBLs were co stimulated by anti CD28 and anti CD80 (B7 1) McAb and acted on the hepatoma cells (BEL 7402) ResultsThe activities of PKC and TPK were significantly higher in the activated T cells than those in the control cells, and were positively related to apoptosis of hepatoma cells ConclusionPKC and TPK play important role on the signal transduction in the activated and proliferating T cells and the induction of apoptosis of hepatoma cells<ekeyword><</em></p><p class='subject'><strong>关 键 词：</strong><a href='/subject/article.aspx?id=87619' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e8%9b%8b%e7%99%bd%e6%bf%80%e9%85%b6%5d' title='蛋白激酶'>蛋白激酶</a> <a href='/zk/search.aspx?key=K%3d%5b%e6%8a%97CD28%2bB7.1%e5%8d%95%e6%8a%97%5d' title='抗CD28+B7.1单抗'>抗CD28+B7.1单抗</a> <a href='/subject/article.aspx?id=4085951' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e7%bb%86%e8%83%9e%e5%87%8b%e4%ba%a1%5d' title='细胞凋亡'>细胞凋亡</a> <a href='/subject/article.aspx?id=593778' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e4%bf%a1%e5%8f%b7%e8%bd%ac%e5%af%bc%5d' title='信号转导'>信号转导</a> <a href='/subject/article.aspx?id=6952991' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e8%82%9d%e7%99%8c%5d' title='肝癌'>肝癌</a> <a href='/subject/article.aspx?id=7601832' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5bPBLS%5d' title='PBLS'>PBLS</a> </p><p class='class'><strong>分 类 号：</strong><a href='/zk/search.aspx?key=C%3dR735.7' title='R735.7'>R735.7</a>[<a href = '/domain/article.aspx?id=503' >医药卫生—肿瘤</a>] </p> </div> <div id="content_wzxg" name="wzxg" class="article-graph"> <object width="695" height="250" classid="clsid:D27CDB6E-AE6D-11cf-96B8-444553540000" id="FlashID"> <param value="../flash/relation.swf" name="movie"> <param value="high" name="quality"> <param value="opaque" name="wmode"> <param value="6.0.65.0" name="swfversion"> <param value="dataUrl=/ajax/articlerelativegraph.ashx?id=4817938" 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