血清和卵泡液中抗精子抗体对体外受精—胚胎移植的影响  

Effect of female antisperm antibodies in serum and follicular fluid on in vitro fertilization embryo transfer

作　　者：宋兰林[1] 全松[1] 陈东红[1] 阎宗合[1] 邢福祺[1] 

机构地区：[1]第一军医大学南方医院妇产科,广东广州510515

出　　处：《免疫学杂志》2001年第1期58-59,68,共3页Immunological Journal

摘　　要：目的评价女性抗精子抗体对体外受精—胚胎移植 (IVF- ET)的影响。方法采用 EL ISA法测定施行 IVF- ET的妇女的血清和卵泡液中抗精子抗体 (As Ab)。 A组 :血清 As Ab阳性 13例 ;B组 :血清及卵泡液 As Ab均阳性 2 9例 ;C组 :As Ab阴性 15 8例。分析 3组受精率、卵裂率、胚胎发育情况及临床妊娠率和流产率的关系。结果 A、B组受精率、卵裂率明显低于 C组 (P<0 .0 1)。移植日 ,A、B组 — 级胚胎率较 C组低 ,IV级胚胎及 2原核受精细胞增加 (P<0 .0 5 )。各组妊娠率及流产率无显著差异 (P>0 .0 5 )。结论 As Ab对 IVF和早期胚胎发育有损害 ,体外培养不能完全清除 As Ab对 IVF-ObjectiveTo evaluate the effect of female antisperm antibodies (AsAb) on in vitro fertilization embryo transfer(IVF ET) MethodsConcentration of AsAb of serum and follicular fluid in women undergoing IVF ET was measured with ELISA Group A:13 women with AsAb positive in serum, Group B: 29 women with AsAb positive in serum and follicular fluid, Group C: 158 women with AsAb negative The IVF rate, cleavage rate, early embryonic development as well as clinical pregnancy and abortion rate were analyzed and compared among the three groups respectively ResultsThe IVF rate and cleavage rate in group A and B decreased significantly as compared with group C ( P <0 01) On the day of embryo transfer,Ⅰ—Ⅱgrade embryo rate in group A and B were significantly lower than that in group C However,gradeⅣand 2PN embryo rates increased significantly as compared with group C ( P <0 05) There were no significant difference in pregnancy and abortion rates among the three groups ( P >0 05) ConclusionAsAb may exert a detrimental effect on IVF and early embryonic development, which can not be overcome completely by improving in vitro culture

关 键 词：体外受精-胚胎移植 血清 卵泡液 抗精子抗体 ELISA 

分 类 号：R711.6[医药卫生—妇产科学]