细胞周期素D?_1、Rb及p16在早期胃癌中的表达及意义  

THE EXPRESSION OF CYCLIN D_1、Rb AND p16 PROTEIN IN EARLY GASTRIC CARCINOMA

作　　者：陈晓黎[1] 王康敏[1] 苏宝山[1] 孙润芹[1] 黄莺[1] 强雷[1] 

机构地区：[1]西安医科大学第二附属医院病理科,西安710004

出　　处：《中国普外基础与临床杂志》2001年第1期29-31,共3页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的　观察细胞周期素D1、Rb及 p16在早期胃癌发生中的作用及相互关系。方法　采用免疫组化SP法对 39例早期胃癌癌旁肠化组织及 34例非癌胃粘膜肠化组织进行对比研究。结果　癌组织中 33/39例 (84 6 % )显示细胞周期素D1过度表达 ,癌旁肠化组织也显示细胞周期素D1过度表达。 12 /39例 p16表达阴性 ,3例呈弱阳性。 2 6例Rb阳性的肿瘤中 ,15例显示 p16不表达或低表达 ,而 9例Rb阴性的肿瘤中则p16显示表达增高。结论　细胞周期素D1是早期胃癌发生过程中常见的分子异常 ,细胞周期素D1的激活及Rb的失活在早期胃癌中可共同存在 ;Rb失活与p16表达之间存在负相关关系 ;早期胃癌的发生可能与细胞周期素D1、p16及Rb负反馈调节环路的异常有关。Objective\ To investigate the expression of cell division regulators p16, Rb and cyclin D 1 in human early gasric carcinoma tissues and their role in tumor transformation and the correlation among p16, Rb and cyclin D 1. Methods\ A comparative study was carried out by using immuno histochemical techniques between the paracarcinomatous intestinal metaplasia of 39 cases of early gatric carcinoma and the non carcinomatous gastric mucosal intestinal metaplasia tissues of 34 cases.Results\ Over expression of cyclin D 1 was determined in 33/39 carcinomatous samples(84.6%) and also in para carcinomatous intestinal metaplasia tissues. p16 was undetectable in 12 of 39 samples. Interestingly, 15 of 26 Rb positive cancers had no or low p16,while 9 Rb negative cancers showed high levels of p16.Conclusion\ The over expression of cyclin D 1 may be a common molecular abnormality and an early molecular event in early gastric carcinoma. Cyclin D 1 over expression and Rb inactivation can co exist in early gastric carcinoma. However, there is a reciprocity between Rb inactivation and p16 expression in early gastric carcinoma. Thus, abnormality in the negative feedback regulatory pathway of cyclin D 1,Rb and p16 may be related to the tumorigenesis in early gastric carcinoma.

关 键 词：早期胃癌 细胞调控因子 免疫组织化学 细胞周期素D1 Rb p16 

分 类 号：R735.2[医药卫生—肿瘤] R730.23[医药卫生—临床医学]