乙型肝炎病毒前C变异体的体外转录和翻译  

Study on in vitro transcription and translation of the precore variant of hepatitis B virus

作　　者：郭亚乒 候金林[1] 骆抗先[1] P.Karayiannis 

机构地区：[1]第一军医大学南方医院感染内科,广东广州510515 [2]Department of Medicine,St Mary's Hospital Medical School

出　　处：《第一军医大学学报》2001年第1期10-11,共2页Journal of First Military Medical University

基　　金：国家自然科学基金!(39600125);军队卫生科研课题资助项目!(962024)

摘　　要：目的明确乙型肝炎病毒（ＨＢＶ）前Ｃ第１７位和２９位密码子突变体对ｅ抗原合成和分泌的影响。方法用ＰＣＲ 方法扩增ＨＢＶ前Ｃ／Ｃ基因区，克隆后测序及进行序列分析。将ＨＢＶ前Ｃ区Ｔ１８６２／Ａ１８９９位点突变体亚克隆人表达 质粒 ｐＧＥＭＴ中，经体外翻译后，比较野毒株和变异株的表达产物。结果该前 Ｃ区突变株在体外仍能合成 ＨＢＶ ｅ前体 蛋白。结论前 Ｃ区 Ｔ１８６２／Ａ１８９９突变并不能阻断 ＨＢＶ ｅ抗原前体蛋白合成，ｅ抗原前体可能在分泌过程中受阻。Objective To investigate the effect of T1862 and A1899 codon mutants of hepatitis B virus (HBV) on the synthesis and excretion of the precursor of hepatitis B e antigen. Methods The entire precore and core region were amplified from the sera of a HBeAg -negative patient with fulminant hepatitis B by polymerase chain reaction (PCR). PCR products were cloned into plasmid pUC 18 and sequenced for analysis of precore mutations.Precore and core sequence of T1862/A 1899 variant were also cloned into expression plasmid pGEMT for in vitro tlanscription and translation study on synthesis and excretion of e antigen. Results T1862 /A1899 variant did not interrupt the synthesis of the precursor of e antigen. Conclusion The absence of e antigen in patients with the folminant hepatitis may be explained by precore codon mutation T1862/A1899, which may exercise its effect on the excretion of the precursor of e antigen, other than the synthesis of precursor protein.

关 键 词：乙型肝炎病毒 体外转录 翻译 乙型肝炎 前C变异体 E抗原 

分 类 号：R512.62[医药卫生—内科学]