甲状腺肿瘤增殖细胞核抗原表达的免疫组化研究  

Immunohistochemical study of proliferating cell nuclear antigen on thyroid tumors

作　　者：孙青[1] 鹿伟[1] 张传森[1] 吴彩志[1] 高钟禹[1] 孙德刚[1] 

机构地区：[1]千佛山医院病理科,山东济南250014

出　　处：《第一军医大学学报》2001年第1期17-18,共2页Journal of First Military Medical University

基　　金：山东省卫生厅医药卫生科研基金!(920021)

摘　　要：目的探讨增殖细胞核抗原（ＰＣＮＡ）表达在甲状腺肿瘤病理学诊断和鉴别诊断中的意义及应用价值。方法采用 免疫组化ＬＳＡＢ法检测１４例甲状腺腺瘤，１０例不典型腺瘤，１５例乳头状腺癌，１０例滤泡性腺癌，１０例髓样癌，１０例未 分化癌。结果 ＰＣＮＡ增殖指数在甲状腺腺瘤为 １２．８，不典型腺瘤为 １９．８，乳头状癌为 １９．２，滤泡性腺癌为 ２６．４，髓样癌 为２７．９，未分化癌为４４．３。各组间比较：不典型腺瘤与乳头状癌之间无差异；腺瘤与不典型腺瘤和乳头状癌间有显著性 差异（Ｐ＜０．０５），与其余各组有极显著性差异（Ｐ＜０．０１）。结论ＰＣＮＡ增殖指数可作为判断甲状腺肿瘤良恶性的指标，并 可反映其临床生物学行为，为预后提供重要信息。Objective To evaluate the significance and practical value of examining the expression of proliferating cell nuclear antigen (PCNA) in the differentiation and diagnosis of thyroid tumors. Methods The expression of PCNA in the tumor tissues in 14 cases of thyroid adenoma, 10 cases of atypical adenoma,15 papillary carcinoma, 10 follicular carcinoma, 10 medullary thyroid carcinoma and 10 undifferentiated carcinoma were determined by immunohistochemical method with strept avidin-biotin (LSAB). Results PCNA proliferating index were 12.8 in thyroid adenoma,19.8 in atypical adenoma, 19.2 in papillary carcinoma, 26.4 follicular carcinoma, 27.9 in medullary thyroid carcinoma and 44.3 in undifferentiated carcinoma. No significant difference in the PCNA proliferating index between thyroid adenoma and papillary carcinoma was observed,while that between thyroid adenoma and atypical adenoid tumors along with various carcinomas was found to be significantly different (p<0.01). Conclusions PCNA proliferating index may be useful indicators for the differentiation and diagnosis of thyroid tumors and for the prognostic assessment.

关 键 词：甲状腺肿瘤 增殖细胞核抗原 免疫组织化学 PCNA 

分 类 号：R736.1[医药卫生—肿瘤] R730.23[医药卫生—临床医学]