白细胞介素(IL)-2与IL-4对小鼠膀胱癌肿瘤浸润性淋巴细胞增殖及细胞毒性的协同调节作用  

The synergy effects of IL-2 and IL-4 on the proliferation and cytotoxicity of tumor infiltrating lymphocytes from murine bladder carcinoma

作　　者：王志勇[1] 于满[1] 李俊悦[2] 周性明[2] 张忠林[2] 

机构地区：[1]河北承德医学院附属医院泌尿外科,067000 [2]南京铁道医学院附属医院泌尿外科

出　　处：《中华实验外科杂志》2001年第1期15-16,共2页Chinese Journal of Experimental Surgery

摘　　要：目的　探讨白细胞介素 (IL) 2与IL 4对膀胱癌肿瘤浸润性淋巴细胞 (TIL)体外增殖及细胞毒性免疫调控的协同作用。方法　分离膀胱癌TIL ,置于含IL 2和 (或 )IL 4的完全培养基中培养 4周 ,定期计数TIL增殖数量。四甲基偶氮唑蓝 (MTT)比色法检测TIL细胞毒性。结果　对比单纯IL 2的培养条件 ,IL 2联合IL 4后 4周时TIL扩增数量是前者的 1.6 5倍 (P <0 .0 5 )。在效靶比为 10∶1时 ,TIL对自体膀胱癌细胞 (BTT739)表现出高水平的杀伤活性 (P <0 .0 5 )。联合培养的TIL抗BTT739或小鼠淋巴瘤瘤株 (YAC 1)的活性与在单纯IL 2培养的条件下相比无显著改变 (P均 >0 .0 5 )。结论　IL 4对IL 2活化的膀胱癌TIL增殖具有较强的正向调节效应 ,而对TIL细胞毒性未见明显影响。Objective To investigate the synergy effects of IL 2 and IL 4 on the proliferation and cytotoxicity of tumor infiltrating lymphocytes (TIL) from murine bladder carcinoma in vitro. Methods Suspensions of TIL were prepared from T739 murine bladder carcinoma.All cultures were incubated in RPMI 1640 complete media with IL 2 and /or IL 4. During 4 weeks of culture,TILs were counted regularly.The cytotoxicity of TILs was measured using MTT colorimetric assay. Results The fold expansions of TILs in IL 2 plus IL 4 for 4 weeks was 1.65 times higher than that in IL 2 alone ( P < 0.05 ).The cytotoxic activity of TIL against autologous BTT739 cells was stronger than that against YAC 1 tumor cell line at an E/T ration of 10∶1( P <0.05).IL 2 in conjunction with IL-4 could not preferentially augment the cytolysis of TIL to BTT739 cancer cells or YAC 1 tumor cell line than TIL growed in IL 2 alone did ( P >0.05 ). Conclusion IL 4 can significantly promote the proliferation of bladder carcinoma TILs activated by IL 2, while exhibite no statistically significant effect on the cytotoxicity of TIL.

关 键 词：肿瘤浸润 白细胞介素-4 白细胞介素-2 膀胱肿瘤 细胞增殖 细胞毒性 

分 类 号：R737.14[医药卫生—肿瘤]