PAR-1激活对小鼠内皮祖细胞CXCR4 mRNA表达及细胞增殖与迁移的影响  

Effect of activated PAR-1 on the expression of CXCR4 mRNA,proliferation and migration of mouse endothelial progenitor cells

作　　者：代静澜[1] 潘波[1] 古平[1] 牟海刚[1] 白家驷[2] 

机构地区：[1]解放军第三二四医院心血管内科,重庆400020 [2]第三军医大学西南医院全军肝病中心实验室,重庆400038

出　　处：《重庆医学》2013年第33期4056-4058,4061,共4页Chongqing medicine

基　　金：解放军第三二四医院院管课题资助项目(2010008)

摘　　要：目的研究蛋白酶激活受体-1(PAR-1)活化后对小鼠内皮祖细胞(EPCs)CXC型趋化因子受体4(CXCR4)mRNA表达及细胞增殖、迁移的影响。方法分离鉴定小鼠EPCs,分别以不同浓度PAR-1激活剂SFLLRN加入培养的EPCs中,以PAR-1小干扰RNA(siRNA)转染EPCs,并设立空白对照组。采用荧光定量实时聚合酶链反应(RT-PCR)分析各组细胞PAR-1、CXCR4mRNA表达;MTT法检测各组EPCs增殖情况;Transwell小室共培养分析各组EPCs的迁移能力。结果 SFLLRN激活组EPCs增殖率、迁移率及PAR-1、CXCR4mRNA表达均较空白对照组、PAR-1特异siRNA转染组增高,差异有统计学意义(P<0.05)。CXCR4mRNA与PAR-1mRNA表达呈明显正相关(r=0.991)。结论 PAR-1可能通过促使CXCR4的表达增加,从而促进EPCs的增殖和迁移。Objective To explore the effect of protease-activated receptor 1（PAR-1） activation on the expression of CXCR4 mR-NA of mouse endothelial progenitor cells （EPCs） and proliferation ,migration of EPCs .Methods Mouse EPCs were activated by different concentration of SFLLRN （one agonist of PAR-1） ,or transfected by small interfering RNA of PAR-1 .The expressions of PAR-1 and CXCR4 mRNA of EPCs were detected by fluorescent quantitative real-time PCR .Proliferation ,migration of EPCs were detected by MTT ,Transwell chambers respectively .Results The expressions of PAR-1 and CXCR4 mRNA in SFLLRN group were higher than that in other groups（P〈 0 .05） .The expression of CXCR4 mRNA was highly positive correlation with PAR-1 mRNA（r=0 .991） .The proliferation ,migration of mouse EPCs were induced by activation of PAR-1 .Conclusion Activation of PAR-1 promotes cell proliferation and migration of mouse EPCs ,this effect may be depended on CXCR4 .

关 键 词：内皮祖细胞 蛋白酶激活受体-1 CXC型趋化因子受体4 细胞增殖 迁移 

分 类 号：R363[医药卫生—病理学]