艾叶水提取物对肝癌细胞的抑制作用及对Bcl-2和HSP70蛋白表达的影响  

Leaves water extract on the liver human hepatocellular carcinoma xenografts and protein expression of Bcl-2 and HSP70

作　　者：陆树桐[1] 詹世林[1] 陈建雄[1] 

机构地区：[1]广州军区广州总医院肝胆外科,广州510000

出　　处：《重庆医学》2013年第33期4062-4063,4066,共3页Chongqing medicine

摘　　要：目的观察艾叶水提取物对H27人肝癌细胞抑制作用及对Bcl-2、热休克蛋白(HSP70)蛋白表达的影响。方法从细胞的形态学观察,并进一步使用免疫细胞化学剂、蛋白免疫印迹法(Western blotting)等分析方法研究癌基因Bcl-2及HSP70蛋白的表达情况。结果 60mg/mL的艾叶水提取物处理肝癌细胞12h后,肝癌细胞均出现严重皱缩、固缩、细胞质膜突出等,免疫组化及Western blotting分析显示实验过程中伴随着癌基因Bcl-2表达的减弱(P<0.05),而HSP70蛋白表达则无明显变化(P>0.05)。结论艾叶水提取物能够诱导体外人肝癌细胞凋亡,而这个凋亡过程可能跟癌基因Bcl-2有关,与HSP70的表达关系不明显。Objective To explore the leaves water extract on the liver human hepatocellular carcinoma xenografts and protein expression of Bcl-2 and HSP70 .Methods From cell morphology and DNA agarose gel electrophoresis the observation leaves water extract the characteristics of the material role in human hepatoma cells ,further use of immunocytochemistry agent Western blotting analysis method oncogene Bcl-2 and tumor suppressor genes HSP70expression .Results After the processing of the the 60 mg/mL leaves water extract of liver cancer cells after 12 h ,liver cells showed severe shrinkage ,pyknosis ,highlight the cell plasma mem-brane ,immunohistochemistry and Western blotting analysis showed that during the experiment along with the oncogene Bcl-2 ex-pression weakened（P〈0 .05） ,while the expression of tumor suppressor genes HSP70 had no obvious change（P〈0 .05） .Conclusion Water extract of the leaves can be induced in vitro human liver cancer cell apoptosis ,and the apoptotic process may be followed by the oncogene Bcl-2 ,tumor suppressor gene expression of HSP70 relationship .

关 键 词：肝肿瘤 HSP70热休克蛋白质类 艾叶水提取物 BCL-2 

分 类 号：R735.7[医药卫生—肿瘤]