顶空气相色谱法测定左亚叶酸钠中离子交换树脂残留溶剂  

Determination of ion exchange resin residual solvents in sodium levofolinate by headspace gas chromatography

作　　者：王杰晶[1] 李银峰[2] 李佩[3] 张园园[3] 靳朝东[2] 

机构地区：[1]河南大学,河南开封475001 [2]天津药物研究院分析测试中心,天津300193 [3]天津中医药大学,天津300193

出　　处：《现代药物与临床》2013年第6期901-903,共3页Drugs & Clinic

摘　　要：目的建立了左亚叶酸钠中8种离子交换树脂有机溶剂残留物的检测方法。方法采用顶空气相色谱法。色谱柱为DB-624毛细管色谱柱（30．0m×0．53mm×3．00μm）；氢火焰离子化检测器；程序升温；载气：氮气；体积流量：2．5mL／min；项空加热温度：90℃；进样量：3mL。结果在该条件下，8种残留溶剂完全分离，且在2～40μg／mL线性关系良好，平均回收率在81．73％～108．20％。结论此法操作简单快捷，灵敏度高，结果准确可信，可用于左亚叶酸钠中离子交换树脂残留溶剂检测。Objective To establish a method for detecting eight residues of organic solvent in sodium levofolinate by ion exchange resin. Methods Headspace gas chromatography with DB-624 capillary column （30.0 m ×0.53 mm, 3.00 μm） and FID detector were used with temperature program. Carder gas was nitrogen, and the flow rate was 2.5 mL/min. The headspace temperature was set at 90 ℃ with the injection volume of 10 μL. Results The organic residues were completely separated. There was a good linearity in the range of 2 - 40 μg/mL. The average recoveries of the eight residues were in the range of 81.73% - 108.20%. Conclusion The method is simple, sensitive, and accurate. It could be used for detecting the residual solvents in sodium levofolinate.

关 键 词：左亚叶酸钠 离子交换树脂 残留溶剂 顶空气相色谱 

分 类 号：R927.2[医药卫生—药学]