围植入期二硫化碳暴露对小鼠子宫组织降钙素和黄体酮水平的影响  

Effect of carbon disulfide exposure during peri-implantation period on levels of calcitonin and progesterone in uterus of mice

作　　者：杨柳[1] 王志萍[1] 

机构地区：[1]山东大学公共卫生学院流行病与卫生统计学系,济南250012

出　　处：《中华劳动卫生职业病杂志》2013年第12期881-885,共5页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金面上项目（81072275）

摘　　要：目的观察二硫化碳（CS2）对孕鼠子宫组织降钙素和黄体酮（P4）水平的影响，探讨围植入期暴露CS2致胚胎植入障碍的作用机制。方法168只健康雌性昆明孕鼠随机分到4种处理的14个观察终点组中，分别在孕3d（GD3）、GD4、GD5、GD6腹腔注射631．4mg／kg剂量的CS2（观察终点的染毒组）或橄榄油（观察终点的对照组）1次；在不同的观察终点（GD4、GD5、GD6、GD7、GD9）结束实验。采用酶联免疫吸附试验（ELISA）检测各观察终点孕鼠子宫组织中降钙素及P4水平。结果胚胎围植入期不同时点暴露于CS2均可导致孕鼠胚胎植入数目减少，GD3、GD4、GD5和GD6染毒组胚胎植入数目分别比对照组减少了42．85％、63．74％、60．45％和47．26％，差异均有统计学意义（P〈0．01）。GD3、GD4、GD5和GD6染毒组各观察终点孕鼠子宫组织中降钙素表达水平下降，差异均有统计学意义（P〈0．05）。GD3、GD4和GD5暴露组孕鼠子宫组织P4水平下降，差异均有统计学意义（R0．05）。GD3、GD4、GD5和GD6染毒组胚胎植入数目与子宫组织降钙素和P4表达水平呈正相关（r=0．670和r=0．632，P〈0．01）；孕鼠子宫组织降钙素表达水平与P4水平呈正相关（r=0．325，P〈0．01）。结论围植入期暴露CS2可降低孕鼠子宫组织降钙素和P4表达水平，可能是CS2致胚胎植入障碍的分子机制之一。Objective To evaluate the effect of carbon disulfide （CS2） exposure during the peri-impla- ntation period on the levels of calcitonin （CT） and progesterone （P4） in the uterus of pregnant mice and to investigate the mechanism of embryo loss induced by CS2 exposure during the peri-implantation period. Methods A total of 168 healthy pregnant Kunming mice were randomly assigned to receive an intraperitoneal injection of CS2 （631.4 mg/kg） or olive oil （control） on gestational day （GD） 3, GD4, GD5, or GD6. The experiment was completed at different end points （GD4, GD5, GD6, GD7, and GD9）. The levels of CT and P4 in the uterus were measured by enzyme-linked immunosorbent assay at each end point. Results The numbers of implanted embryos in GD3, GD4, GD5, and GD6 exposure groups significantly decreased by 42.85%, 63.74%, 60.45%, and 47.26%, respectively, compared with those in control group （P〈0.01）. The GD3, GD4, GD5, and GD6 exposure groups had significantly decreased CT levels at each end point （P〈0.05）, and the GD3, GD4, and GD5 exposure groups had significantly decreased P4 levels （P〈0.05）. In the GD3, GD4, GDS, and GD6 exposure groups, the number of implanted embryos was positively related with the levels of CT and P4 expressed in the uterus （r= 0.670, P〈0.01; r=0.632, P〈0.01 ）; the expression level of CT was positively related with that of P4 in the uterus of pregnant mice （r=0.325, P〈0.01）. Conclusion Exposure to CS2 during the peri-implantation period can reduce the expression levels of CT and P4 in the uterus of pregnant mice, which might be one of the molecular mechanisms of embryo loss induced by CS2 exposure.

关 键 词：二硫化碳 胚胎植入 降钙素 黄体酮 

分 类 号：R114[医药卫生—卫生毒理学]