肺癌组织中AKAP95与细胞周期蛋白D1和E1及临床病理学指标的关系  

Relationship between AKAP95, cyclin El, cyclin D1, and clinicopathological parameters in lung cancer tissue

作　　者：胡素贤[1] 孔祥余 原阳阳[4] 滕伯刚[4] 陟雪红 庄文鑫 于修义[2] 刘文志[3] 张永兴[4] 

机构地区：[1]厦门大学附属第一医院呼吸内科,361003 [2]厦门大学附属第一医院胸外科,361003 [3]大连大学附属中山医 [4]厦门大学公共卫生学院

出　　处：《中华劳动卫生职业病杂志》2013年第12期890-894,共5页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金资助项目（81071927）;福建省自然科学基金资助项目（2009J01199）;福建省医学创新课题资助计划项目（2012-CXB-25）;厦门大学基础创新科研基金（本科生项目）（CXB2013）

摘　　要：目的探讨PKA的锚定蛋白A—kinaseanchorprotein95（AKAP95）、细胞周期蛋白CyclinEl蛋白和CyclinDl蛋白在肺癌组织中表达的关联性。方法应用免疫组化方法检测51例肺癌组织中AKAP95、CyclinEl和CyclinDl蛋白的表达。结果1．CyclinEl蛋白在肺癌中的表达水平明显高于癌旁组织，阳性率分别为75．56％和20．00％，差异有统计学意义（P〈0．01）；其表达与组织学分型、淋巴结是否发生转移和分化程度均无关，差异无统计学意义（P〉0．05）；2．CyclinDl蛋白在肺癌组织表达的阳性率为69．39％，高于癌旁组织的表达阳性率为（14．29％），其表达与组织学分型有关，差异有统计学意义（P〈0．05），3．AKAP95和CyclinEl蛋白、AKAP95和CyclinDl蛋白在肺癌组织中均呈关联性表达，差异有统计学意义（P〈0．01）。结论1．CyclinEl和CyclinDl蛋白在肺癌组织中高表达，提示在肺癌的发生发展中发挥作用；2．CyclinEl蛋白表达与肺癌的分化程度、组织学分型及淋巴结转移均无关；CyclinDl蛋白表达与组织学分型有关；3．AKAP95和CyclinEl蛋白、AKAP95和CyclinDl蛋白在肺癌组织中均呈关联性表达。Objective To investigate the correlation between expression of A-kinase anchoring protein 95 （AKAP95） and protein expression of cyclin E1 and cyclin D1 in lung cancer tissue. Methods Fifty-one cases of lung cancer were included in the study. The protein expression of AKAP95, cyclin El, and cyclin D1 were measured by immunohistochemistry. Results The protein expression of cyclin E1 in lung cancer tissues was significantly higher than that in paracancerous tissues （positive rate： 75.56% vs 20%, P〈0.01）; its expression showed no relationship with histopathological type, lymph node metastasis, and cellular differentiation （P〉0.05）. The protein expression of cyclin D1 in lung cancer tissues was higher than that in paracancerous tissues （positive rate： 69.39% vs 14.29%）; its expression showed a significant relationship with histopathological type （P〈0.05）. The expression of AKAP95 was correlated with the protein expression of cyclin E1 and cyclin D1 in lung cancer tissues （P〈0.01）. Conclusion Cyclin E1 and cyclin D1 are highly expressed in lung cancer tissue, suggesting that they play an important role in the development and progression of lung cancer. The protein expression of cyclin E1 has no relationship with cellular differentiation, lymph node metastasis, and histopathological type of lung cancer, and the protein expression of cyclin D1 has a significant relationship with histopathological type. The expression of AKAP95 is correlated with the protein expression of cyclin E1 and cyclin D1 in lung cancer tissue.

关 键 词：肺癌 AKAP95 CyclinE1 CYCLIND1 关联性分析 

分 类 号：R734.2[医药卫生—肿瘤]