转基因耐草甘膦除草剂玉米CC-2喂养SD大鼠90天亚慢性毒性研究  

A 90-day Subchronic Feeding Study of Transgenic Glyphosate Herbicide-resistant Maize CC-2 in Sprague-Dawley Rats (Rattus norvegicus)

作　　者：陈德龙[1] 张莳眉 邹世颖[1] 刘一非[1] 周忻[1,2] 贺晓云[1,2] 黄昆仑[1,2] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083 [2]农业部转基因生物食用安全监督检验测试中心,北京100083

出　　处：《农业生物技术学报》2013年第12期1448-1457,共10页Journal of Agricultural Biotechnology

基　　金：转基因生物新品种培育重大专项(No.2013ZX08011-006)

摘　　要：为了评估转基因耐除草剂玉米中长期喂养Sprague-Dawley(SD)大鼠的安全性,本研究将转基因耐草甘膦除草剂玉米(CC-2)及其非转基因对照玉米(郑-58)分别以12.5%、25%和50%的比例添加到基础饲料中,饲喂SD大鼠90 d,检测其对SD大鼠营养状况和生理指标的影响,实验期间,观察各组动物的体重、血常规以及血生化指标,实验末期解剖动物进行大体病理观察,计算脏器系数。结果显示,转基因玉米喂养动物90 d后,各组动物生长发育良好,转基因玉米组与非转基因玉米对照组相比,SD大鼠的血生化、血常规、脏器系数等个别指标有统计学差异(P<0.05),但这些差异无剂量相关性,无生物学意义。研究表明,长期饲喂转基因耐除草剂玉米CC-2与非转基因玉米对SD大鼠具有同样的安全性。本研究为转基因玉米的安全应用提供了科学数据支持。To evaluate the safety of transgenic herbicide-resistant maize, the transgenic maize and its non-transgenic counterpart were fed to Sprague-Dawley（SD） rats（Rattus norvegicus） during 90 d. This study added the genetically modified（GM） maize（CC-2） and its non-transgenic control maize（Zheng-58） in a ratio of 12.5%, 25.0% and 50.0% respectively to the basal feed. The influence and safety of GM maize were evaluated by monitoring SD rats＇ nutrition, physiological status, and biochemical indicators. During the 90 d feeding for SD rats, we surveyed the nutritional status of SD rats and the effects of physiological indexes, such as body weight, hematology and serum biochemistry indexes. At the end of the study, we dissected the animals for pathological observation and calculated viscera coefficient. After 90 days, animals of each group were in good condition. Compared with the non-GM maize control group, several indexes of the serum biochemistry, hematology, relative organ weight of SD rats fed on GM corn had statistically significant difference（P〈0.05） and no dose-relative or gender-relative. So it had no biological significance. The transgenic herbicide-resistant maize is as same safety as its non-GM maize in 90 d feeding study. This study provides scientific evidence for the safe usage of GM maize.

关 键 词：耐除草剂 转基因玉米 草甘膦 SD大鼠 90d喂养实验 

分 类 号：S513[农业科学—作物学]