婆罗双树样基因4在人前列腺癌细胞和组织中的表达及其与临床预后之间的关系  

Expression of SAL-like 4 protein in human prostate cancer tissue/cell lines and its relationship with clinical outcome

作　　者：赖义明 黄海[1] 凌逸虹[2] 曾乐祥[3] 陈杰青[4] 曹亿[5] 余永晟 张思敏[1] 张一鸣[1] 李金[1] 王淦平[1] 郭正辉[1] 

机构地区：[1]中山大学孙逸仙纪念医院泌尿外科,广东广州510120 [2]中山大学肿瘤防治中心病理科,广州510060 [3]中山大学孙逸仙纪念医院小儿外科,广东广州510120 [4]深圳市第二人民医院泌尿外科,广东深圳518035 [5]中山大学孙逸仙纪念医院急诊外科,广东广州510120 [6]增城市人民医院泌尿外科,广东广州511300

出　　处：《中国病理生理杂志》2013年第12期2186-2192,共7页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.81272807;No.81101947);广东省自然科学基金资助项目(No.S2011010004546;No.S2012010010780;No.10151008901000070);广东省科技社会发展项目(No.2012B032000006)

摘　　要：目的:评估婆罗双树样基因4(SALL4)在人前列腺癌细胞系和前列腺癌组织中的表达情况,并明确其表达水平和临床病理学参数间的关系。方法:用免疫荧光技术、RT-PCR和Western blotting检测SALL4在LNCaP、DU145、PC-3细胞系和RWPE-1正常前列腺上皮细胞系中的表达。同时用免疫组化方法检测SALL4在前列腺增生及前列腺癌组织中的表达,并探讨其与Gleason评分等临床病理参数的关系。结果:SALL4蛋白在细胞中主要表达于胞浆,在3种前列腺癌细胞株中SALL4蛋白表达水平要明显高于正常前列腺上皮细胞RWPE-1(P<0.05),而SALL4mRNA表达水平在4种细胞系中无明显差异(P>0.05)。免疫组化结果显示SALL4在前列腺癌组织中的表达水平明显高于增生和正常前列腺组织(P<0.01)。SALL4蛋白表达水平与Gleason评分、前列腺癌临床分期、预后及组织前列腺特异抗原(PSA)表达密切相关,而与患者年龄、治疗前血清总PSA水平、前列腺体积及组织雄激素受体表达无明显相关性。结论:SALL4蛋白在前列腺癌中高表达,提示其在前列腺癌的发生、发展过程中可能起重要作用,有可能成为诊断前列腺癌新的肿瘤标志物及评估其恶性程度、进展和预后的参考指标。AIM ： To evaluate the expression of SAL-like 4 （SALL4） protein in human prostate cancer cell lines and tissues, and to analyze the relationship between SALL4 expression and the clinicopathological parameters. METHODS： Immunofluorescence, RT-PCR and Western blotting were performed to detect the expression of SALL4 at mRNA and protein levels in 3 common prostate cancer cell lines LNCaP, DU145 and PC-3. The normal prostate epithelial cell line RWPE-1 was used for control. The protein levels of SALL4 in the tissues of benign prostate hyperplasia and pros- tate cancer tissues were determined by the method of immunohistochemistry. RESULTS ： The SALIA protein was predomi- nantly expressed in the cytoplasm of the cells. The protein levels of SALL4 in 3 common prostate cancer cell lines were sig-nificantly higher than that in RWPE-1 cells. However, the mRNA level of SALL4 had no obvious difference among the 4 cell lines. Immunohistochemistry results showed that the expression level of SALL4 in the cancerous tissues was significant- ly higher than that in noncancerous （benign and normal） prostatic tissues. In addition, we found that the expression level of SALL4 in prostate cancer was significantly correlated with the Gleason score, clinical stage, prognosis estimation and tis- sue prostate-specific antigen （PSA） expression, but not associated with age, the level of serum total PSA, prostate volume and the expression of androgen receptor in the tissues of the patients. CONCLUSION： The over-expression of SALL4 pro- tein may play an important role in the pathogenesis and progression of prostate cancer, and provides some reference indexes for estimating the malignancy, progression and prognosis of prostate cancer.

关 键 词：SALL4蛋白 前列腺肿廇 前列腺特异抗原 

分 类 号：R363[医药卫生—病理学]