抗环瓜氨酸肽抗体与类风湿关节炎患者成纤维样滑膜细胞迁移和侵袭能力的相关研究  

Relationship between anti-CCP antibody and migration or invasion ability of fibroblast-like synoviocytes from rheumatoid arthritis patients

作　　者：刘志昌[1] 肖游君[1] 劳敏曦[1] 黄明城[1] 曾珊[1] 许韩师[1] 杨岫岩[1] 梁柳琴[1] 

机构地区：[1]中山大学附属第一医院风湿免疫内科,广东广州510080

出　　处：《中国病理生理杂志》2013年第12期2277-2281,2291,共6页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.81072484);广东省科技计划(No.2010B080701099;No.2011B080701011);广东省教育厅创新项目(No.2012KJCX0003)

摘　　要：目的:研究抗环瓜氨酸肽(anti-cyclic citrullinated peptide,CCP)抗体与类风湿关节炎(rheumatoid arthritis,RA)患者成纤维样滑膜细胞(fibroblast-like synoviocyte,FLS)迁移和侵袭的关系。方法:FLS分离自22例活动性RA患者、12例骨关节炎(OA)和6例无关节炎病史的外伤患者滑膜组织;FLS迁移和侵袭能力采用Transwell小室测定。结果:RA组、OA组和正常组FLS迁移数目分别为(29.33±10.93)、(9.28±7.87)和(7.00±4.07)个/视野,侵袭细胞数分别为(14.35±7.67)、(3.96±4.37)和(3.08±1.03)个/视野,RA组与其它2组的迁移细胞数和侵袭细胞数比较均有显著差异;抗CCP抗体阳性RA患者FLS与抗CCP阴性RA患者FLS相比,前者迁移和侵袭能力均强于后者;相关性分析亦发现,抗CCP抗体阳性及抗CCP抗体滴度与RA患者FLS的侵袭和迁移能力密切相关。结论:抗CCP抗体阳性的RA患者FLS的迁移和侵袭能力更强,抗CCP抗体可能与RA患者FLS的迁移和侵袭能力改变有关。AIM： To investigate the relationship between anti-cyclic citrullinated peptide （anti-CCP） antibody and migration or invasion ability of fibroblast-like synoviocytes （FLS） from rheumatoid arthritis （RA） patients. METH- ODS： FLS was isolated from 22 patients with active RA, 12 patients with osteoarthritis （OA） and 6 patients with joint in- juries without arthritis history. The migration and invasion abilities were assessed by Transwell assay. RESULTS： Among the patients of RA, OA and normal controls, the numbers of migratory cells were （29.33 ± 10.93）, （9.28 ±7.87） and （ 7.00 ± 4.07 ）/field, respectively, and the numbers of invasive cells were （ 14.35 ± 7.67）, （ 3.96 ± 4.37） and （3.08 ± 1.03）/field, respectively. The numbers of migratory and invasive cells were higher in RA than those in the other groups. The migration and invasion abilities of RA FLS from anti-CCP antibody-positive RA patients were significantly increased as compared with anti-CCP antibody-negative RA patients. Correlation analysis revealed that the migration and invasion abili- ties of RA FLS were correlated with the positive and high titer of anti-CCP antibody. CONCLUSION： The migration and invasion abilities of FLS from anti-CCP antibody-positive RA patients are increased. Anti-CCP antibody is possibly correla- ted with the increased migration and invasion abilities of RA FLS.

关 键 词：关节炎 类风湿 抗环瓜氨酸肽抗体 成纤维样滑膜细胞 细胞迁移 

分 类 号：R363[医药卫生—病理学]